Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation

Eric V. Granowitz, Noriko Tonomura, Rita M. Benson, Deborah M. Katz, Vimla Band, Grace P. Makari-Judson, Barbara A. Osborne

Research output: Contribution to journalArticle

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Abstract

Background: Hyperbaric oxygenation (HBO) therapy is the administration of 100%-inhaled oxygen to patients at increased atmospheric pressure. Materials and Methods: We used an in vitro model to examine the effects of HBO on mammary cell proliferation. Normal mammary epithelia, primary tumor and metastatic tumor cells derived from the same patient and immortalized by transfection with the human papilloma virus E6 oncogene, as well as the MCF7 human mammary adenocarcinoma cell line, were studied. Results: HBO (97.9% O2, 2.1% CO2, 2.4 atmospheres absolute) inhibited the proliferation of all 4 cell types as measured by light microscopy, [3H]thymidine uptake, a tetrazolium-based colorimetric assay and a clonogenicity assay. The anti-proliferative effect of HBO was time-dependent (p<0.01 for all 4 cell types). Hyperoxia alone (95% O2, 5% CO2, 1 atmosphere absolute) and increased atmospheric pressure alone (8.75% O2, 2.1% CO2, 2.4 atmospheres absolute) also inhibited proliferation, but their effects were not as profound as HBO (p<0.01 when either hyperoxia or increased pressure was compared to HBO for all 4 cell types). HBO enhanced the anti-proliferative effects of melphalan (p<0.05), gemcitabine (p<0.001) and paclitaxel (p<0.001). The clonogenicity assay demonstrated that the effects of HBO were still evident 2 weeks after the exposure (p<0.01 for all 4 cell types). Experiments using Hoechst-propidium iodide or annexin V-propidium iodide staining showed no HBO-induced increases in necrosis or apoptosis. Conclusion: HBO inhibits benign and malignant mammary epithelial cell proliferation, but does not enhance cell death.

Original languageEnglish (US)
Pages (from-to)3833-3842
Number of pages10
JournalAnticancer Research
Volume25
Issue number6 B
StatePublished - Nov 1 2005

Fingerprint

Hyperbaric Oxygenation
Breast
Epithelial Cells
Cell Proliferation
Oxygen
Atmosphere
Hyperoxia
Atmospheric Pressure
Propidium
gemcitabine
Papillomaviridae
Melphalan
Annexin A5
Paclitaxel
Oncogenes
Thymidine
Transfection
Microscopy
Neoplasms
Adenocarcinoma

Keywords

  • Breast
  • Epithelial cells
  • Hyperbaric oxygenation
  • Mammary neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Granowitz, E. V., Tonomura, N., Benson, R. M., Katz, D. M., Band, V., Makari-Judson, G. P., & Osborne, B. A. (2005). Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation. Anticancer Research, 25(6 B), 3833-3842.

Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation. / Granowitz, Eric V.; Tonomura, Noriko; Benson, Rita M.; Katz, Deborah M.; Band, Vimla; Makari-Judson, Grace P.; Osborne, Barbara A.

In: Anticancer Research, Vol. 25, No. 6 B, 01.11.2005, p. 3833-3842.

Research output: Contribution to journalArticle

Granowitz, EV, Tonomura, N, Benson, RM, Katz, DM, Band, V, Makari-Judson, GP & Osborne, BA 2005, 'Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation', Anticancer Research, vol. 25, no. 6 B, pp. 3833-3842.
Granowitz EV, Tonomura N, Benson RM, Katz DM, Band V, Makari-Judson GP et al. Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation. Anticancer Research. 2005 Nov 1;25(6 B):3833-3842.
Granowitz, Eric V. ; Tonomura, Noriko ; Benson, Rita M. ; Katz, Deborah M. ; Band, Vimla ; Makari-Judson, Grace P. ; Osborne, Barbara A. / Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation. In: Anticancer Research. 2005 ; Vol. 25, No. 6 B. pp. 3833-3842.
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abstract = "Background: Hyperbaric oxygenation (HBO) therapy is the administration of 100{\%}-inhaled oxygen to patients at increased atmospheric pressure. Materials and Methods: We used an in vitro model to examine the effects of HBO on mammary cell proliferation. Normal mammary epithelia, primary tumor and metastatic tumor cells derived from the same patient and immortalized by transfection with the human papilloma virus E6 oncogene, as well as the MCF7 human mammary adenocarcinoma cell line, were studied. Results: HBO (97.9{\%} O2, 2.1{\%} CO2, 2.4 atmospheres absolute) inhibited the proliferation of all 4 cell types as measured by light microscopy, [3H]thymidine uptake, a tetrazolium-based colorimetric assay and a clonogenicity assay. The anti-proliferative effect of HBO was time-dependent (p<0.01 for all 4 cell types). Hyperoxia alone (95{\%} O2, 5{\%} CO2, 1 atmosphere absolute) and increased atmospheric pressure alone (8.75{\%} O2, 2.1{\%} CO2, 2.4 atmospheres absolute) also inhibited proliferation, but their effects were not as profound as HBO (p<0.01 when either hyperoxia or increased pressure was compared to HBO for all 4 cell types). HBO enhanced the anti-proliferative effects of melphalan (p<0.05), gemcitabine (p<0.001) and paclitaxel (p<0.001). The clonogenicity assay demonstrated that the effects of HBO were still evident 2 weeks after the exposure (p<0.01 for all 4 cell types). Experiments using Hoechst-propidium iodide or annexin V-propidium iodide staining showed no HBO-induced increases in necrosis or apoptosis. Conclusion: HBO inhibits benign and malignant mammary epithelial cell proliferation, but does not enhance cell death.",
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AU - Makari-Judson, Grace P.

AU - Osborne, Barbara A.

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