Hydrophobized antiviral antibodies and antisense oligonucleotides

E. S. Severin, N. S. Melik-Nubarov, A. V. Ovcharenko, S. V. Vinogradov, V. I. Kiselev, A. V. Kabanov

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A method of suppressing virus reproduction in cells has been proposed. The approach consists of affecting the cells with antiviral antibodies artificially hydrophobized with fatty acid residues. Reproduction of influenza viruses in MDCK cells and respiratory-synticial virus in HeLa cells was used as a model to demonstrate that poly- and monoclonal antibodies, modified by 1 or 2 stearic acid residues, are potent, unlike the non-modified antibodies, at inhibiting viral reproduction. The observed phenomenon is apparently due to penetration of hydrophobized antibodies into the cells. Thus, in particular, considerable antiviral activity is exhibited by monoclonal antibodies against NP-protein of influenza virus, which is an antigen accessible to antibodies only inside the infected cells. Hydrophobized antibodies do not affect the kinetics of viral protein synthesis; they block the virus withdrawal from the cells, probably by interfering with the assembling and budding of virus particles. To enhance penetration of oligonucleotides ("oligos") into cells, chemical modification of the former at the 5′-end phosphate group by fatty radicals has been suggested. The undecanol-modified oligo namely an oligo complementary to the protein binding sites located at the influenza virus polymerases encoding RNA, was synthesized using a DNA-synthesator. The above modified oligo effectively suppressed the influenza A/PR8/34 virus reproduction and inhibited synthesis of the virus-specific proteins in MDCK cells. The non-modified antisense oligo and the modified nonsense oligo did not affect the virus development under the same conditions.

Original languageEnglish (US)
Pages (from-to)417-426,IN25-IN26,427-430
JournalAdvances in Enzyme Regulation
Volume31
Issue numberC
DOIs
StatePublished - 1991

Fingerprint

Antisense Oligonucleotides
Viruses
Antiviral Agents
Reproduction
Antibodies
Orthomyxoviridae
Madin Darby Canine Kidney Cells
Monoclonal Antibodies
Viral Proteins
DNA-Directed RNA Polymerases
HeLa Cells
Protein Binding
Oligonucleotides
Virion
Human Influenza
Proteins
Fatty Acids
Phosphates
Binding Sites
Chemical modification

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Severin, E. S., Melik-Nubarov, N. S., Ovcharenko, A. V., Vinogradov, S. V., Kiselev, V. I., & Kabanov, A. V. (1991). Hydrophobized antiviral antibodies and antisense oligonucleotides. Advances in Enzyme Regulation, 31(C), 417-426,IN25-IN26,427-430. https://doi.org/10.1016/0065-2571(91)90027-J

Hydrophobized antiviral antibodies and antisense oligonucleotides. / Severin, E. S.; Melik-Nubarov, N. S.; Ovcharenko, A. V.; Vinogradov, S. V.; Kiselev, V. I.; Kabanov, A. V.

In: Advances in Enzyme Regulation, Vol. 31, No. C, 1991, p. 417-426,IN25-IN26,427-430.

Research output: Contribution to journalArticle

Severin, ES, Melik-Nubarov, NS, Ovcharenko, AV, Vinogradov, SV, Kiselev, VI & Kabanov, AV 1991, 'Hydrophobized antiviral antibodies and antisense oligonucleotides', Advances in Enzyme Regulation, vol. 31, no. C, pp. 417-426,IN25-IN26,427-430. https://doi.org/10.1016/0065-2571(91)90027-J
Severin ES, Melik-Nubarov NS, Ovcharenko AV, Vinogradov SV, Kiselev VI, Kabanov AV. Hydrophobized antiviral antibodies and antisense oligonucleotides. Advances in Enzyme Regulation. 1991;31(C):417-426,IN25-IN26,427-430. https://doi.org/10.1016/0065-2571(91)90027-J
Severin, E. S. ; Melik-Nubarov, N. S. ; Ovcharenko, A. V. ; Vinogradov, S. V. ; Kiselev, V. I. ; Kabanov, A. V. / Hydrophobized antiviral antibodies and antisense oligonucleotides. In: Advances in Enzyme Regulation. 1991 ; Vol. 31, No. C. pp. 417-426,IN25-IN26,427-430.
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