Hydrogen peroxide probes directed to different cellular compartments

Mikalai Malinouski, You Zhou, Vsevolod V. Belousov, Dolph L. Hatfield, Vadim N. Gladyshev

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Background: Controlled generation and removal of hydrogen peroxide play important roles in cellular redox homeostasis and signaling. We used a hydrogen peroxide biosensor HyPer, targeted to different compartments, to examine these processes in mammalian cells. Principal Findings: Reversible responses were observed to various redox perturbations and signaling events. HyPer expressed in HEK 293 cells was found to sense low micromolar levels of hydrogen peroxide. When targeted to various cellular compartments, HyPer occurred in the reduced state in the nucleus, cytosol, peroxisomes, mitochondrial intermembrane space and mitochondrial matrix, but low levels of the oxidized form of the biosensor were also observed in each of these compartments, consistent with a low peroxide tone in mammalian cells. In contrast, HyPer was mostly oxidized in the endoplasmic reticulum. Using this system, we characterized control of hydrogen peroxide in various cell systems, such as cells deficient in thioredoxin reductase, sulfhydryl oxidases or subjected to selenium deficiency. Generation of hydrogen peroxide could also be monitored in various compartments following signaling events. Conclusions: We found that HyPer can be used as a valuable tool to monitor hydrogen peroxide generated in different cellular compartments. The data also show that hydrogen peroxide generated in one compartment could translocate to other compartments. Our data provide information on compartmentalization, dynamics and homeostatic control of hydrogen peroxide in mammalian cells.

Original languageEnglish (US)
Article numbere14564
JournalPloS one
Volume6
Issue number1
DOIs
StatePublished - Feb 3 2011

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Hydrogen Peroxide
hydrogen peroxide
biosensors
Cells
Biosensing Techniques
cells
Biosensors
Oxidation-Reduction
Thioredoxin-Disulfide Reductase
Peroxisomes
HEK293 Cells
Peroxides
peroxisomes
Selenium
peroxides
cytosol
Endoplasmic Reticulum
Cytosol
endoplasmic reticulum
selenium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Malinouski, M., Zhou, Y., Belousov, V. V., Hatfield, D. L., & Gladyshev, V. N. (2011). Hydrogen peroxide probes directed to different cellular compartments. PloS one, 6(1), [e14564]. https://doi.org/10.1371/journal.pone.0014564

Hydrogen peroxide probes directed to different cellular compartments. / Malinouski, Mikalai; Zhou, You; Belousov, Vsevolod V.; Hatfield, Dolph L.; Gladyshev, Vadim N.

In: PloS one, Vol. 6, No. 1, e14564, 03.02.2011.

Research output: Contribution to journalArticle

Malinouski, M, Zhou, Y, Belousov, VV, Hatfield, DL & Gladyshev, VN 2011, 'Hydrogen peroxide probes directed to different cellular compartments', PloS one, vol. 6, no. 1, e14564. https://doi.org/10.1371/journal.pone.0014564
Malinouski, Mikalai ; Zhou, You ; Belousov, Vsevolod V. ; Hatfield, Dolph L. ; Gladyshev, Vadim N. / Hydrogen peroxide probes directed to different cellular compartments. In: PloS one. 2011 ; Vol. 6, No. 1.
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