Hyaluronan synthase of chlorella virus PBCV-1

Paul L. DeAngelis, Wei Jing, Michael V. Graves, Dwight E. Burbank, James L Van Etten

Research output: Contribution to journalReview article

96 Citations (Scopus)

Abstract

Sequence analysis of the 330-kilobase genome of the virus PBCV-1 that infects a chlorella-like green algae revealed an open reading frame, A98R, with similarity to several hyaluronan synthases. Hyaluronan is an essential polysaccharide found in higher animals as well as in a few pathogenic bacteria. Expression of the A98R gene product in Escherichia coli indicated that the recombinant protein is an authentic hyaluronan synthase. A98R is expressed early in PBCV-1 infection and hyaluronan is produced in infected algae. These results demonstrate that a virus can encode an enzyme capable of synthesizing a carbohydrate polymer and that hyaluronan exists outside of animals and their pathogens.

Original languageEnglish (US)
Pages (from-to)1800-1803
Number of pages4
JournalScience
Volume278
Issue number5344
DOIs
StatePublished - Dec 5 1997

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Chlorella
Hyaluronic Acid
Viruses
Chlorophyta
Recombinant Proteins
Open Reading Frames
Polysaccharides
Sequence Analysis
Polymers
Carbohydrates
Genome
Escherichia coli
Bacteria
Gene Expression
Enzymes
Infection
hyaluronan synthase

ASJC Scopus subject areas

  • General

Cite this

DeAngelis, P. L., Jing, W., Graves, M. V., Burbank, D. E., & Van Etten, J. L. (1997). Hyaluronan synthase of chlorella virus PBCV-1. Science, 278(5344), 1800-1803. https://doi.org/10.1126/science.278.5344.1800

Hyaluronan synthase of chlorella virus PBCV-1. / DeAngelis, Paul L.; Jing, Wei; Graves, Michael V.; Burbank, Dwight E.; Van Etten, James L.

In: Science, Vol. 278, No. 5344, 05.12.1997, p. 1800-1803.

Research output: Contribution to journalReview article

DeAngelis, PL, Jing, W, Graves, MV, Burbank, DE & Van Etten, JL 1997, 'Hyaluronan synthase of chlorella virus PBCV-1', Science, vol. 278, no. 5344, pp. 1800-1803. https://doi.org/10.1126/science.278.5344.1800
DeAngelis PL, Jing W, Graves MV, Burbank DE, Van Etten JL. Hyaluronan synthase of chlorella virus PBCV-1. Science. 1997 Dec 5;278(5344):1800-1803. https://doi.org/10.1126/science.278.5344.1800
DeAngelis, Paul L. ; Jing, Wei ; Graves, Michael V. ; Burbank, Dwight E. ; Van Etten, James L. / Hyaluronan synthase of chlorella virus PBCV-1. In: Science. 1997 ; Vol. 278, No. 5344. pp. 1800-1803.
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