Hyaluronan Synthase Elevation in Metastatic Prostate Carcinoma Cells Correlates with Hyaluronan Surface Retention, a Prerequisite for Rapid Adhesion to Bone Marrow Endothelial Cells

Melanie A. Simpson, Jane Reiland, Scott R. Burger, Leo T. Furcht, Andrew P. Spicer, Theodore R. Oegema, James B. McCarthy

Research output: Contribution to journalArticle

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Abstract

Bone marrow is the primary site of metastasis in patients with advanced stage prostate cancer. Prostate carcinoma cells metastasizing to bone must initially adhere to endothelial cells in the bone marrow sinusoids. In this report, we have modeled that interaction in vitro using two bone marrow endothelial cell (BMEC) lines and four prostate adenocarcinoma cell lines to investigate the adhesion mechanism. Highly metastatic PC3 and PC3M-LN4 cells were found to adhere rapidly and specifically (70-90%) to BMEC-1 and trHBMEC bone marrow endothelial cells, but not to human umbilical vein endothelial cells (15-25%). Specific adhesion to BMEC-1 and trHBMEC was dependent upon the presence of a hyaluronan (HA) pericellular matrix assembled on the prostate carcinoma cells. DU145 and LNCaP cells were only weakly adherent and retained no cell surface HA. Maximal BMEC adhesion and RA encapsulation were associated with high levels of HA synthesis by the prostate carcinoma cells. Up-regulation of HA synthase isoforms Has2 and Has3 relative to levels expressed by normal prostate corresponded to elevated HA synthesis and avid BMEC adhesion. These results support a model in which tumor cells with up-regulated HA synthase expression assemble a cell surface hyaluronan matrix that promotes adhesion to bone marrow endothelial cells. This interaction could contribute to preferential bone metastasis by prostate carcinoma cells.

Original languageEnglish (US)
Pages (from-to)17949-17957
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number21
DOIs
StatePublished - Jan 25 2001

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Endothelial cells
Hyaluronic Acid
Bone Marrow Cells
Prostate
Bone
Adhesion
Endothelial Cells
Cells
Carcinoma
Cell Adhesion
Cell adhesion
Bone Marrow
Neoplasm Metastasis
hyaluronan synthase
Bone and Bones
Cell Line
Human Umbilical Vein Endothelial Cells
Prostatic Neoplasms
Protein Isoforms
Adenocarcinoma

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hyaluronan Synthase Elevation in Metastatic Prostate Carcinoma Cells Correlates with Hyaluronan Surface Retention, a Prerequisite for Rapid Adhesion to Bone Marrow Endothelial Cells. / Simpson, Melanie A.; Reiland, Jane; Burger, Scott R.; Furcht, Leo T.; Spicer, Andrew P.; Oegema, Theodore R.; McCarthy, James B.

In: Journal of Biological Chemistry, Vol. 276, No. 21, 25.01.2001, p. 17949-17957.

Research output: Contribution to journalArticle

Simpson, Melanie A. ; Reiland, Jane ; Burger, Scott R. ; Furcht, Leo T. ; Spicer, Andrew P. ; Oegema, Theodore R. ; McCarthy, James B. / Hyaluronan Synthase Elevation in Metastatic Prostate Carcinoma Cells Correlates with Hyaluronan Surface Retention, a Prerequisite for Rapid Adhesion to Bone Marrow Endothelial Cells. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 21. pp. 17949-17957.
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