Human Ubc9 contributes to production of fully infectious human immunodeficiency virus type 1 virions

Tareq Jaber, Christopher R. Bohl, Gentry L. Lewis, Charles Wood, John T West, Robert A. Weldon

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Ubc9 was identified as a cellular protein that interacts with the Gag protein of Mason-Pfizer monkey virus. We show here that Ubc9 also interacts with the human immunodeficiency virus type 1 (HIV-1) Gag protein and that their interaction is important for virus replication. Gag was found to colocalize with Ubc9 predominantly at perinuclear puncta. While cells in which Ubc9 expression was suppressed with RNA interference produced normal numbers of virions, these particles were 8- to 10-fold less infectious than those produced in the presence of Ubc9. The nature of this defect was assayed for dependence on Ubc9 during viral assembly, trafficking, and Env incorporation. The Gag-mediated assembly of virus particles and protease-mediated processing of Gag and Gag-Pol were unchanged in the absence of Ubc9. However, the stability of the cell-associated Env glycoprotein was decreased and Env incorporation into released virions was altered. Interestingly, overexpression of the Ubc9 trans-dominant-negative mutant C93A, which is a defective E2-SUMO-1 conjugase, suggests that this activity may not be required for interaction with Gag, virion assembly, or infectivity. This finding demonstrates that Ubc9 plays an important role in the production of infectious HIV-1 virions.

Original languageEnglish (US)
Pages (from-to)10448-10459
Number of pages12
JournalJournal of virology
Volume83
Issue number20
DOIs
StatePublished - Oct 1 2009

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Human immunodeficiency virus 1
virion
Virion
HIV-1
Human Immunodeficiency Virus gag Gene Products
Mason-Pfizer monkey virus
gamma-Glutamyl Hydrolase
env Gene Products
gag Gene Products
Virus Assembly
proteins
Virus Replication
virus replication
RNA Interference
RNA interference
glycoproteins
Peptide Hydrolases
proteinases
pathogenicity
cells

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Human Ubc9 contributes to production of fully infectious human immunodeficiency virus type 1 virions. / Jaber, Tareq; Bohl, Christopher R.; Lewis, Gentry L.; Wood, Charles; West, John T; Weldon, Robert A.

In: Journal of virology, Vol. 83, No. 20, 01.10.2009, p. 10448-10459.

Research output: Contribution to journalArticle

Jaber, Tareq ; Bohl, Christopher R. ; Lewis, Gentry L. ; Wood, Charles ; West, John T ; Weldon, Robert A. / Human Ubc9 contributes to production of fully infectious human immunodeficiency virus type 1 virions. In: Journal of virology. 2009 ; Vol. 83, No. 20. pp. 10448-10459.
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