Human Type III Collagen Gene Expression Is Coordinately Modulated with the Type I Collagen Genes during Fibroblast Growth

Marcelle Miskulin, Raymond Dalgleish, Barbara Kluve-Beckerman, Stephen I. Rennard, Mark Brantly, Ronald G. Crystal, Paul Tolstoshev

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Abstract

Type III collagen is one of the major interstitial collagens and, as such, plays an important role in modulating the structure and function of most tissues. To compare the expression of the type III collagen gene to that of the type I collagen α 1(I) and α2(I) genes, cDNAs encoding the 3’ one-third of the human α1(III) collagen mRNA were obtained by screening a human fetal lung fibroblast cDNA library with a cloned segment of the chicken αl(III) gene. Northern blot analysis of human fetal lung fibroblast RNA demonstrated two αl(III)-specific mRNAs of sizes 6.6 and 5.8 kilobases, sizes clearly different from those of the type I collagen mRNAs. Analyses of populations of dividing and nondividing human lung fibroblasts revealed that, on a per cell basis, the nondividing population contained twice as much αl(III) mRNA than did the dividing population. The same was true for the type I collagen αl(I) and α2(I) mRNA transcripts. Similar results were obtained when αl(III), α1(I), and α2(I) mRNA transcripts were quantified by using dot blot evaluation of total RNA, Northern analysis of total RNA, and dot blot evaluation of cytoplasmic RNA. Thus, despite the fact that the αl(III) collagen gene is located on a chromosome different from the αl(I) and α2(I) genes, the expression of these three collagen chains appears to be coordinately controlled during periods of rapid and slow fibroblast growth.

Original languageEnglish (US)
Pages (from-to)1408-1413
Number of pages6
JournalBiochemistry
Volume25
Issue number6
DOIs
StatePublished - Jan 1 1986

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Collagen Type III
Fibroblasts
Collagen Type I
Gene expression
Genes
Gene Expression
Messenger RNA
Collagen
Growth
RNA
Lung
Population
Gene encoding
Chromosomes
Gene Library
Northern Blotting
Chickens
Screening
Complementary DNA
Tissue

ASJC Scopus subject areas

  • Biochemistry

Cite this

Miskulin, M., Dalgleish, R., Kluve-Beckerman, B., Rennard, S. I., Brantly, M., Crystal, R. G., & Tolstoshev, P. (1986). Human Type III Collagen Gene Expression Is Coordinately Modulated with the Type I Collagen Genes during Fibroblast Growth. Biochemistry, 25(6), 1408-1413. https://doi.org/10.1021/bi00354a033

Human Type III Collagen Gene Expression Is Coordinately Modulated with the Type I Collagen Genes during Fibroblast Growth. / Miskulin, Marcelle; Dalgleish, Raymond; Kluve-Beckerman, Barbara; Rennard, Stephen I.; Brantly, Mark; Crystal, Ronald G.; Tolstoshev, Paul.

In: Biochemistry, Vol. 25, No. 6, 01.01.1986, p. 1408-1413.

Research output: Contribution to journalArticle

Miskulin, M, Dalgleish, R, Kluve-Beckerman, B, Rennard, SI, Brantly, M, Crystal, RG & Tolstoshev, P 1986, 'Human Type III Collagen Gene Expression Is Coordinately Modulated with the Type I Collagen Genes during Fibroblast Growth', Biochemistry, vol. 25, no. 6, pp. 1408-1413. https://doi.org/10.1021/bi00354a033
Miskulin M, Dalgleish R, Kluve-Beckerman B, Rennard SI, Brantly M, Crystal RG et al. Human Type III Collagen Gene Expression Is Coordinately Modulated with the Type I Collagen Genes during Fibroblast Growth. Biochemistry. 1986 Jan 1;25(6):1408-1413. https://doi.org/10.1021/bi00354a033
Miskulin, Marcelle ; Dalgleish, Raymond ; Kluve-Beckerman, Barbara ; Rennard, Stephen I. ; Brantly, Mark ; Crystal, Ronald G. ; Tolstoshev, Paul. / Human Type III Collagen Gene Expression Is Coordinately Modulated with the Type I Collagen Genes during Fibroblast Growth. In: Biochemistry. 1986 ; Vol. 25, No. 6. pp. 1408-1413.
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