Human Skeletal Muscle mRNA Response to a Single Hypoxic Exercise Bout

Dustin R Slivka, Matthew W.S. Heesch, Charles L. Dumke, John S. Cuddy, Walter S. Hailes, Brent C. Ruby

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Background The ability to physically perform at high altitude may require unique strategies to acclimatize before exposure. The effect of acute hypoxic exposure on the metabolic response of the skeletal muscle may provide insight into the value of short-term preacclimatization strategies. Objective To determine the human skeletal muscle response to a single acute bout of exercise in a hypoxic environment on metabolic gene expression. Methods Eleven recreationally active male participants (24 ± 4 years, 173 ± 20 cm, 82 ± 12 kg, 15.2 ± 7.1% fat, 4.0 ± 0.6 L/min maximal oxygen consumption) completed two 1-hour cycling exercise trials at 60% of peak power followed by 4 hours of recovery in ambient environmental conditions (975 m) and at normobaric hypoxic conditions simulating 3000 m in a randomized counterbalanced order. Muscle biopsies were obtained from the vastus lateralis before exercise and 4 hours after exercise for real-time polymerase chain reaction analysis of select metabolic genes. Results Gene expression of hypoxia-inducible factor 1 alpha, cytochrome c oxidase subunit 4, peroxisome proliferator-activated receptor gamma coactivator 1 alpha, hexokinase, phosphofructokinase, mitochondrial fission 1, and mitofusin-2 increased with exercise (P <.05) but did not differ with hypoxic exposure (P >.05). Optic atrophy 1 did not increase with exercise or differ between environmental conditions (P >.05). Conclusions The improvements in mitochondrial function reported with intermittent hypoxic training may not be explained by a single acute hypoxic exposure, and thus it appears that a longer period of preacclimatization than a single exposure may be required.

    Original languageEnglish (US)
    Pages (from-to)462-465
    Number of pages4
    JournalWilderness and Environmental Medicine
    Volume25
    Issue number4
    DOIs
    StatePublished - Dec 1 2014

    Fingerprint

    Skeletal Muscle
    Autosomal Dominant Optic Atrophy
    Mitochondrial Dynamics
    Gene Expression
    Hypoxia-Inducible Factor 1
    Phosphofructokinases
    Messenger RNA
    Hexokinase
    Quadriceps Muscle
    Electron Transport Complex IV
    Oxygen Consumption
    Real-Time Polymerase Chain Reaction
    Fats
    Exercise
    Biopsy
    Muscles
    Genes
    Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha

    Keywords

    • HIF-1α
    • PGC-1α
    • altitude exposure
    • glycolytic enzymes
    • mRNA
    • mitochondria

    ASJC Scopus subject areas

    • Emergency Medicine
    • Public Health, Environmental and Occupational Health

    Cite this

    Human Skeletal Muscle mRNA Response to a Single Hypoxic Exercise Bout. / Slivka, Dustin R; Heesch, Matthew W.S.; Dumke, Charles L.; Cuddy, John S.; Hailes, Walter S.; Ruby, Brent C.

    In: Wilderness and Environmental Medicine, Vol. 25, No. 4, 01.12.2014, p. 462-465.

    Research output: Contribution to journalArticle

    Slivka, Dustin R ; Heesch, Matthew W.S. ; Dumke, Charles L. ; Cuddy, John S. ; Hailes, Walter S. ; Ruby, Brent C. / Human Skeletal Muscle mRNA Response to a Single Hypoxic Exercise Bout. In: Wilderness and Environmental Medicine. 2014 ; Vol. 25, No. 4. pp. 462-465.
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    abstract = "Background The ability to physically perform at high altitude may require unique strategies to acclimatize before exposure. The effect of acute hypoxic exposure on the metabolic response of the skeletal muscle may provide insight into the value of short-term preacclimatization strategies. Objective To determine the human skeletal muscle response to a single acute bout of exercise in a hypoxic environment on metabolic gene expression. Methods Eleven recreationally active male participants (24 ± 4 years, 173 ± 20 cm, 82 ± 12 kg, 15.2 ± 7.1{\%} fat, 4.0 ± 0.6 L/min maximal oxygen consumption) completed two 1-hour cycling exercise trials at 60{\%} of peak power followed by 4 hours of recovery in ambient environmental conditions (975 m) and at normobaric hypoxic conditions simulating 3000 m in a randomized counterbalanced order. Muscle biopsies were obtained from the vastus lateralis before exercise and 4 hours after exercise for real-time polymerase chain reaction analysis of select metabolic genes. Results Gene expression of hypoxia-inducible factor 1 alpha, cytochrome c oxidase subunit 4, peroxisome proliferator-activated receptor gamma coactivator 1 alpha, hexokinase, phosphofructokinase, mitochondrial fission 1, and mitofusin-2 increased with exercise (P <.05) but did not differ with hypoxic exposure (P >.05). Optic atrophy 1 did not increase with exercise or differ between environmental conditions (P >.05). Conclusions The improvements in mitochondrial function reported with intermittent hypoxic training may not be explained by a single acute hypoxic exposure, and thus it appears that a longer period of preacclimatization than a single exposure may be required.",
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    AU - Heesch, Matthew W.S.

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    AU - Hailes, Walter S.

    AU - Ruby, Brent C.

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    N2 - Background The ability to physically perform at high altitude may require unique strategies to acclimatize before exposure. The effect of acute hypoxic exposure on the metabolic response of the skeletal muscle may provide insight into the value of short-term preacclimatization strategies. Objective To determine the human skeletal muscle response to a single acute bout of exercise in a hypoxic environment on metabolic gene expression. Methods Eleven recreationally active male participants (24 ± 4 years, 173 ± 20 cm, 82 ± 12 kg, 15.2 ± 7.1% fat, 4.0 ± 0.6 L/min maximal oxygen consumption) completed two 1-hour cycling exercise trials at 60% of peak power followed by 4 hours of recovery in ambient environmental conditions (975 m) and at normobaric hypoxic conditions simulating 3000 m in a randomized counterbalanced order. Muscle biopsies were obtained from the vastus lateralis before exercise and 4 hours after exercise for real-time polymerase chain reaction analysis of select metabolic genes. Results Gene expression of hypoxia-inducible factor 1 alpha, cytochrome c oxidase subunit 4, peroxisome proliferator-activated receptor gamma coactivator 1 alpha, hexokinase, phosphofructokinase, mitochondrial fission 1, and mitofusin-2 increased with exercise (P <.05) but did not differ with hypoxic exposure (P >.05). Optic atrophy 1 did not increase with exercise or differ between environmental conditions (P >.05). Conclusions The improvements in mitochondrial function reported with intermittent hypoxic training may not be explained by a single acute hypoxic exposure, and thus it appears that a longer period of preacclimatization than a single exposure may be required.

    AB - Background The ability to physically perform at high altitude may require unique strategies to acclimatize before exposure. The effect of acute hypoxic exposure on the metabolic response of the skeletal muscle may provide insight into the value of short-term preacclimatization strategies. Objective To determine the human skeletal muscle response to a single acute bout of exercise in a hypoxic environment on metabolic gene expression. Methods Eleven recreationally active male participants (24 ± 4 years, 173 ± 20 cm, 82 ± 12 kg, 15.2 ± 7.1% fat, 4.0 ± 0.6 L/min maximal oxygen consumption) completed two 1-hour cycling exercise trials at 60% of peak power followed by 4 hours of recovery in ambient environmental conditions (975 m) and at normobaric hypoxic conditions simulating 3000 m in a randomized counterbalanced order. Muscle biopsies were obtained from the vastus lateralis before exercise and 4 hours after exercise for real-time polymerase chain reaction analysis of select metabolic genes. Results Gene expression of hypoxia-inducible factor 1 alpha, cytochrome c oxidase subunit 4, peroxisome proliferator-activated receptor gamma coactivator 1 alpha, hexokinase, phosphofructokinase, mitochondrial fission 1, and mitofusin-2 increased with exercise (P <.05) but did not differ with hypoxic exposure (P >.05). Optic atrophy 1 did not increase with exercise or differ between environmental conditions (P >.05). Conclusions The improvements in mitochondrial function reported with intermittent hypoxic training may not be explained by a single acute hypoxic exposure, and thus it appears that a longer period of preacclimatization than a single exposure may be required.

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    KW - PGC-1α

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    KW - glycolytic enzymes

    KW - mRNA

    KW - mitochondria

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