Human sera obtained from patients with cataract killed lens epithelial cells in culture

L. T. Chylack, D. P. Singh, T. Kikuchi, T. Sueno, J. Wolfe, T. Shinohara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: The broad goal of this study is to assess the importance of autoimmunity in the formation of human cataract. Methods: Patients (N=365) were examined once using LOCSIII. Serum was analyzed for antibody to lens antigens using ELISA and protein blot analysis against human central lens epithelial cell antigens. A subset of these serum samples was analyzed for toxicity to mouse and human lens epithelial cells in vitro. Results: All patients had detectable levels of anti-lens antibody. There was no association between the antibody level and the type or LOCSIII grade for any type of cataract. There was a significant association between the LOCSIII grade of cortical opacification and the presence of a band at approximately 21 kDa on the immune blot. All patients had detectable levels of anti-lens antibody. Ninety six percent of sera from cataractous patients (147/153), and 21 percent of sera from non-cataract individuals (9/43) killed mouse lens epithelial cells in culture. A majority of sera from cataract patients killed 60-100% of mouse lens epithelial cells, and the sera from non-cataract patients killed 0-10% of the lens cells. To isolate the cidal factors, we fractionated sera with high cidal potency by molecular sieve and affinity column chromatographies and found that they were immunoglobulins (IgG and/or IgM). Conclusions: There is also a strong association between the presence of age-related cataract and the cytotoxicity of serum to lens epithelial cells. The cytotoxic antibodies were either IgG or IgM.

Original languageEnglish (US)
Pages (from-to)S1142
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

Fingerprint

Cataract
Lenses
Cell Culture Techniques
Epithelial Cells
Serum
Immunoglobulin M
Antibodies
Anti-Idiotypic Antibodies
Immunoglobulin G
Antigens
Autoimmunity
Affinity Chromatography
Immunoglobulins
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Human sera obtained from patients with cataract killed lens epithelial cells in culture. / Chylack, L. T.; Singh, D. P.; Kikuchi, T.; Sueno, T.; Wolfe, J.; Shinohara, T.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996, p. S1142.

Research output: Contribution to journalArticle

Chylack, LT, Singh, DP, Kikuchi, T, Sueno, T, Wolfe, J & Shinohara, T 1996, 'Human sera obtained from patients with cataract killed lens epithelial cells in culture', Investigative Ophthalmology and Visual Science, vol. 37, no. 3, pp. S1142.
Chylack, L. T. ; Singh, D. P. ; Kikuchi, T. ; Sueno, T. ; Wolfe, J. ; Shinohara, T. / Human sera obtained from patients with cataract killed lens epithelial cells in culture. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3. pp. S1142.
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