Human respiratory tract secretions. Mucous glycoproteins of nonpurulent tracheobronchial secretions, and sputum of patients with bronchitis and cystic fibrosis

Thomas F. Boat, Pi-Wan Cheng, Rama N. Iyer, Don M. Carlson, Ilona Polony

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Mucous glycoproteins were isolated by agarose gel filtration from nonpurulent tracheobronchial secretions and purulent sputum which had been reduced, carboxymethylated and, in the case of purulent secretions, treated with deoxyribonuclease. The solubilized and purified glycoproteins were fractionated on diethylaminoethyl cellulose into two major (I, II) and two minor (Ia, III) blood group active components. Components I and II had similar carbohydrate and amino acid compositions which were typical for human blood group substances. These two components did differ in several respects. Component I contained 1.4-2.6% sulfate and did not inhibit influenza virus hemagglutination while component II contained 7.1-7.8% sulfate and was a potent inhibitor of virus hemagglutination. Component II also migrated more rapidly on sodium dodecyl sulfate-3.3% acrylamide gel electrophoresis. Components I and II in purulent secretions displayed only minor compositional differences from their counterparts in nonpurulent secretions. Component II was more abundant in two sputum samples from subjects with cystic fibrosis than in purulent bronchitic secretions or in nonpurulent secretions.

Original languageEnglish (US)
Pages (from-to)95-104
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume177
Issue number1
DOIs
StatePublished - Jan 1 1976

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Bronchitis
Hemagglutination
Blood Group Antigens
Sputum
Viruses
Cystic Fibrosis
Respiratory System
Sulfates
Glycoproteins
Gels
Deoxyribonucleases
Acrylamide
Orthomyxoviridae
Electrophoresis
Cellulose
Sodium Dodecyl Sulfate
Sepharose
Gel Chromatography
Carbohydrates
Amino Acids

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Human respiratory tract secretions. Mucous glycoproteins of nonpurulent tracheobronchial secretions, and sputum of patients with bronchitis and cystic fibrosis. / Boat, Thomas F.; Cheng, Pi-Wan; Iyer, Rama N.; Carlson, Don M.; Polony, Ilona.

In: Archives of Biochemistry and Biophysics, Vol. 177, No. 1, 01.01.1976, p. 95-104.

Research output: Contribution to journalArticle

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