62 Citations (Scopus)

Abstract

Summary. We previously reported detection of human parvovirus B19 DNA in livers from patients requiring transplantation for acute fulminant liver failure. In this study, we used immune adherence PCR (IA-PCR) to bind B19 virions in recipient native liver onto solid phase with specific monoclonal antibodies followed by PCR amplification of virion DNA. IA-PCR had sensitivity and specificity similar to conventional PCR. We examined liver tissue from 16 patients with non-A, non-B, non-C, non-E (NA-E) acute fulminant liver failure (AFLF) (6 of unknown etiology associated with aplastic anemia (AA), 4 of unknown etiology without AA; and 6 patients with AFLF of known etiology). IA-PCR detected B19 virions in 5 of 6 (83%) of livers from patients with idiopathic NA-E AFLF associated with AA and in 2 of 3 (75%) without AA, compared to 1 of 6 (17%) of livers from patients with AFLF of known etiology and to 6 of 34 (18%) of 34 control patients with chronic or neoplastic liver disease. Viral mRNA encoding the structural protein was detected in the liver tissue from three B19 IA-PCR positive patients with AFLF. Detection of B19 virions and mRNA for capsid proteins provided strong evidence for B19 infection during the course of NA-E AFLF and argues for involvement of B19 virus in liver injury.

Original languageEnglish (US)
Pages (from-to)1713-1724
Number of pages12
JournalArchives of Virology
Volume144
Issue number9
DOIs
StatePublished - Oct 29 1999

Fingerprint

Human Parvovirus B19
Parvoviridae Infections
Acute Liver Failure
Aplastic Anemia
Liver
Virion
Polymerase Chain Reaction
Messenger RNA
DNA
Capsid Proteins

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Human parvovirus B19 infection in acute fulminant liver failure. / Karetnyi, Y. V.; Beck, P. R.; Markin, Rodney Smith; Langnas, Alan Norman; Naides, S. J.

In: Archives of Virology, Vol. 144, No. 9, 29.10.1999, p. 1713-1724.

Research output: Contribution to journalArticle

Karetnyi, Y. V. ; Beck, P. R. ; Markin, Rodney Smith ; Langnas, Alan Norman ; Naides, S. J. / Human parvovirus B19 infection in acute fulminant liver failure. In: Archives of Virology. 1999 ; Vol. 144, No. 9. pp. 1713-1724.
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