Human papillomavirus in penile squamous cell lesions

A comparison of an isotopic RNA and two commercial nonisotopic DNA in situ hybridization methods

A. S. Masih, M. H. Stoler, G. M. Farrow, Sonny Lennart Johansson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Sensitive isotopic in situ hybridization analysis of 34 paraffin-embedded penile squamous cell lesions for human papillomavirus (HPV) types 6, 11, 16, 18, and 31 revealed similar HPV detection rates, HPV-type specificity, and viral distribution patterns to those described in analagous cervical and vulvar lesions. Human papillomavirus was detected in six of six cases of condylomata acuminata (HPV type 6 [n=4], HPV type 11 [n=2]), in six of eight cases of squamous cell carcinoma in situ (HPV type 16 [n=5], HPV type 31 [n=1]), and in four of 10 cases of squamous cell carcinoma (all were HPV type 16). Interestingly, all 10 cases of penile verrucous carcinoma analyzed were HPV negative. Reevaluation of the HPV-positive penile lesions with two commercial nonisotopic HPV-typing in situ hybridization kits (Pathogene, Enzo, New York, NY; Viratype, Life Technologies, Gaithersburg, Md), revealed positive results for HPV type 6 and/or type 11 in all six cases of condylomata acuminata studied. However, only the Viratype assay detected HPV genome in the high-grade squamous cell lesions, with a relative sensitivity of 70% compared with that of the isotopic assay.

Original languageEnglish (US)
Pages (from-to)302-307
Number of pages6
JournalArchives of Pathology and Laboratory Medicine
Volume117
Issue number3
StatePublished - Jan 1 1993

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In Situ Hybridization
Epithelial Cells
RNA
Human papillomavirus 6
DNA
Human papillomavirus 31
Human papillomavirus 11
Condylomata Acuminata
Human papillomavirus 16
Squamous Cell Carcinoma
Verrucous Carcinoma
Carcinoma in Situ
Human Genome
Paraffin
Technology

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Human papillomavirus in penile squamous cell lesions : A comparison of an isotopic RNA and two commercial nonisotopic DNA in situ hybridization methods. / Masih, A. S.; Stoler, M. H.; Farrow, G. M.; Johansson, Sonny Lennart.

In: Archives of Pathology and Laboratory Medicine, Vol. 117, No. 3, 01.01.1993, p. 302-307.

Research output: Contribution to journalArticle

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