Human leukocyte antigen class II associations in patients with idiopathic dilated cardiomyopathy

Maria D. Lozano, Ronald J. Rubocki, Janet E. Wilson, Bruce M. McManus, James Lowell Wisecarver

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Idiopathic dilated cardiomyopathy (IDC) is a disease of unknown etiology for which immune abnormalities, possibly related to viral infections, are suspected but unproven. Previous serologic studies have reported associations between human leukocyte antigen DR4 and IDC. A molecular study of human leukocyte antigen associations was undertaken in patients with IDC to further explore the possibility of susceptibility markers of genetically determined disease. Methods and Results: In this study, 36 patients from the Myocarditis Treatment Trial (32 IDC and 4 myocarditis patients) were examined using restriction fragment length polymorphism analysis and polymerase chain reaction amplification with sequence-specific primers to perform class II typing. All 4 myocarditis patients were DQ5 positive and 3 possessed the allele DQB1*0501. In the IDC group, the frequency of human leukocyte antigen DR4 was similar to that reported in the normal population. In addition, there was no excess prevalence of any molecularly defined DR4 allclcs (0401-0419). There was an increase in the frequency of DR12 in IDC patients. The frequencies of the alleles DQB1 *0503 and DQB1*0301 and/or *0304 were also increased in IDC patients versus the normal population. Conclusion: The molecular studies point to a relationship between the DQ locus and IDC.

Original languageEnglish (US)
Pages (from-to)97-103
Number of pages7
JournalJournal of Cardiac Failure
Volume3
Issue number2
DOIs
StatePublished - Jan 1 1997

Fingerprint

Dilated Cardiomyopathy
HLA Antigens
Myocarditis
Virus Diseases
Gene Frequency
Restriction Fragment Length Polymorphisms
Population
Alleles
Polymerase Chain Reaction

Keywords

  • Cardiac diseases
  • DQ locus
  • Myocarditis
  • Polymerase chain reaction amplification with sequence-specific primers

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Human leukocyte antigen class II associations in patients with idiopathic dilated cardiomyopathy. / Lozano, Maria D.; Rubocki, Ronald J.; Wilson, Janet E.; McManus, Bruce M.; Wisecarver, James Lowell.

In: Journal of Cardiac Failure, Vol. 3, No. 2, 01.01.1997, p. 97-103.

Research output: Contribution to journalArticle

Lozano, Maria D. ; Rubocki, Ronald J. ; Wilson, Janet E. ; McManus, Bruce M. ; Wisecarver, James Lowell. / Human leukocyte antigen class II associations in patients with idiopathic dilated cardiomyopathy. In: Journal of Cardiac Failure. 1997 ; Vol. 3, No. 2. pp. 97-103.
@article{6e2f73066701458c8fbdc0be09700548,
title = "Human leukocyte antigen class II associations in patients with idiopathic dilated cardiomyopathy",
abstract = "Background: Idiopathic dilated cardiomyopathy (IDC) is a disease of unknown etiology for which immune abnormalities, possibly related to viral infections, are suspected but unproven. Previous serologic studies have reported associations between human leukocyte antigen DR4 and IDC. A molecular study of human leukocyte antigen associations was undertaken in patients with IDC to further explore the possibility of susceptibility markers of genetically determined disease. Methods and Results: In this study, 36 patients from the Myocarditis Treatment Trial (32 IDC and 4 myocarditis patients) were examined using restriction fragment length polymorphism analysis and polymerase chain reaction amplification with sequence-specific primers to perform class II typing. All 4 myocarditis patients were DQ5 positive and 3 possessed the allele DQB1*0501. In the IDC group, the frequency of human leukocyte antigen DR4 was similar to that reported in the normal population. In addition, there was no excess prevalence of any molecularly defined DR4 allclcs (0401-0419). There was an increase in the frequency of DR12 in IDC patients. The frequencies of the alleles DQB1 *0503 and DQB1*0301 and/or *0304 were also increased in IDC patients versus the normal population. Conclusion: The molecular studies point to a relationship between the DQ locus and IDC.",
keywords = "Cardiac diseases, DQ locus, Myocarditis, Polymerase chain reaction amplification with sequence-specific primers",
author = "Lozano, {Maria D.} and Rubocki, {Ronald J.} and Wilson, {Janet E.} and McManus, {Bruce M.} and Wisecarver, {James Lowell}",
year = "1997",
month = "1",
day = "1",
doi = "10.1016/S1071-9164(97)90041-5",
language = "English (US)",
volume = "3",
pages = "97--103",
journal = "Journal of Cardiac Failure",
issn = "1071-9164",
publisher = "Churchill Livingstone",
number = "2",

}

TY - JOUR

T1 - Human leukocyte antigen class II associations in patients with idiopathic dilated cardiomyopathy

AU - Lozano, Maria D.

AU - Rubocki, Ronald J.

AU - Wilson, Janet E.

AU - McManus, Bruce M.

AU - Wisecarver, James Lowell

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Background: Idiopathic dilated cardiomyopathy (IDC) is a disease of unknown etiology for which immune abnormalities, possibly related to viral infections, are suspected but unproven. Previous serologic studies have reported associations between human leukocyte antigen DR4 and IDC. A molecular study of human leukocyte antigen associations was undertaken in patients with IDC to further explore the possibility of susceptibility markers of genetically determined disease. Methods and Results: In this study, 36 patients from the Myocarditis Treatment Trial (32 IDC and 4 myocarditis patients) were examined using restriction fragment length polymorphism analysis and polymerase chain reaction amplification with sequence-specific primers to perform class II typing. All 4 myocarditis patients were DQ5 positive and 3 possessed the allele DQB1*0501. In the IDC group, the frequency of human leukocyte antigen DR4 was similar to that reported in the normal population. In addition, there was no excess prevalence of any molecularly defined DR4 allclcs (0401-0419). There was an increase in the frequency of DR12 in IDC patients. The frequencies of the alleles DQB1 *0503 and DQB1*0301 and/or *0304 were also increased in IDC patients versus the normal population. Conclusion: The molecular studies point to a relationship between the DQ locus and IDC.

AB - Background: Idiopathic dilated cardiomyopathy (IDC) is a disease of unknown etiology for which immune abnormalities, possibly related to viral infections, are suspected but unproven. Previous serologic studies have reported associations between human leukocyte antigen DR4 and IDC. A molecular study of human leukocyte antigen associations was undertaken in patients with IDC to further explore the possibility of susceptibility markers of genetically determined disease. Methods and Results: In this study, 36 patients from the Myocarditis Treatment Trial (32 IDC and 4 myocarditis patients) were examined using restriction fragment length polymorphism analysis and polymerase chain reaction amplification with sequence-specific primers to perform class II typing. All 4 myocarditis patients were DQ5 positive and 3 possessed the allele DQB1*0501. In the IDC group, the frequency of human leukocyte antigen DR4 was similar to that reported in the normal population. In addition, there was no excess prevalence of any molecularly defined DR4 allclcs (0401-0419). There was an increase in the frequency of DR12 in IDC patients. The frequencies of the alleles DQB1 *0503 and DQB1*0301 and/or *0304 were also increased in IDC patients versus the normal population. Conclusion: The molecular studies point to a relationship between the DQ locus and IDC.

KW - Cardiac diseases

KW - DQ locus

KW - Myocarditis

KW - Polymerase chain reaction amplification with sequence-specific primers

UR - http://www.scopus.com/inward/record.url?scp=0031154637&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031154637&partnerID=8YFLogxK

U2 - 10.1016/S1071-9164(97)90041-5

DO - 10.1016/S1071-9164(97)90041-5

M3 - Article

C2 - 9220309

AN - SCOPUS:0031154637

VL - 3

SP - 97

EP - 103

JO - Journal of Cardiac Failure

JF - Journal of Cardiac Failure

SN - 1071-9164

IS - 2

ER -