Human immunodeficiency virus type l (H1V-1) gp120-induced increases in reactive oxygen species in human U937 cells

I. O. Foga, A. Nath, B. Hasinoff, J. D. Geiger

Research output: Contribution to journalArticle

Abstract

H3V-1 coat protein gp!20 appears to help mediate viral entry and viral tropism, and have neurotoxic properties. Qne mechanism for neurotoxicity may be gp!20-induced formation of reactive oxygen species (ROS). Using a thiobarbituric acid assay, we determined, in human myelomonocytic U937 cells, the extent to which ROS were formed by recombinant gp!20 (H1V-13B) and, for comparison, hypoxanthine/xanthine (H/X). gp!20 was as effective as H/X at inducing ROS production; EC50 value for gp!20 was approximately 11 pM. Co-incubation of gp!20 with polyclonal antibody to gp!20 significantly (p<0.05) inhibited gp!20-induced ROS production by 42%. gp!20- and H/X-induced ROS production were almost equally blocked by a variety of antioxidants and free radical scavengers. gp!20induced ROS production was significantly (pO.Ol) inhibited 78% by nondialyzed and 100% by dialyzed catalase/superoxide dismutase (1000/300 units/ml), 78% by 50 uM desferrioxamine (p<0.01), 53% by 40 uM Vitamin E (p<0.05), and 82% by 100 uM EDTA (p<0.01). The nucleoside transport inhibitor dipyridamole (Persantin™) significantly (p<0.05) inhibited gp!20-induced ROS production at concentrations as low as 0.3 μM; apparent IC50 value was 1.0 μM.

Original languageEnglish (US)
Pages (from-to)A131
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

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U937 Cells
Human immunodeficiency virus
Viruses
reactive oxygen species
Reactive Oxygen Species
HIV
xanthine
hypoxanthine
Hypoxanthine
Xanthine
cells
Dipyridamole
Viral Tropism
dipyridamole
deferoxamine
tropisms
Free Radical Scavengers
Deferoxamine
neurotoxicity
nucleosides

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Human immunodeficiency virus type l (H1V-1) gp120-induced increases in reactive oxygen species in human U937 cells. / Foga, I. O.; Nath, A.; Hasinoff, B.; Geiger, J. D.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996, p. A131.

Research output: Contribution to journalArticle

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