Human immune response to Campylobacter jejuni proteins expressed in vivo

P. Panigrahi, G. Losonsky, L. J. DeTolla, J. G. Morris

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Campylobacter jejuni 81-176 grown in vivo in rabbit ileal loops expresses novel proteins that are not expressed under standard laboratory culture conditions. A new protein with a molecular mass of ca. 180 kDa is expressed at 14, 24, and 48 h of infection. Three other proteins, with molecular masses of ca. 66, 43, and 35 kDa, are overexpressed during different phases of infection. Expression of these proteins stops immediately during the first passage in laboratory media, and they do not elicit a human immune response. Two other proteins, with molecular masses of ca. 84 and 47 kDa, expressed 48 h after infection can be identified by using convalescent sera from human volunteers who were immune to C. jejuni infection upon rechallenge; these proteins were not visualized on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels by Coomassie blue staining or silver staining. Antibodies to the 84- and 47-kDa proteins are of the immunoglobulin G class. Both preinfection and convalescent human sera react strongly to the C. jejuni flagellin (a 58-kDa protein), suggesting the presence of cross-reactive antibodies to this protein in healthy humans. Major outer membrane protein and flagella may play a role in providing protection against C. jejuni disease, but our data suggest that there are other proteins expressed only during in vivo growth of the organism that elicit a strong immune response in human C. jejuni infections.

Original languageEnglish (US)
Pages (from-to)4938-4944
Number of pages7
JournalInfection and immunity
Volume60
Issue number11
StatePublished - Jan 1 1992

Fingerprint

Campylobacter jejuni
Proteins
Campylobacter Infections
Infection
Flagellin
Silver Staining
Flagella
Antibodies
Immunoglobulin Isotypes
Serum
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Volunteers
Membrane Proteins
Immunoglobulin G
Gels
Staining and Labeling
Rabbits

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Panigrahi, P., Losonsky, G., DeTolla, L. J., & Morris, J. G. (1992). Human immune response to Campylobacter jejuni proteins expressed in vivo. Infection and immunity, 60(11), 4938-4944.

Human immune response to Campylobacter jejuni proteins expressed in vivo. / Panigrahi, P.; Losonsky, G.; DeTolla, L. J.; Morris, J. G.

In: Infection and immunity, Vol. 60, No. 11, 01.01.1992, p. 4938-4944.

Research output: Contribution to journalArticle

Panigrahi, P, Losonsky, G, DeTolla, LJ & Morris, JG 1992, 'Human immune response to Campylobacter jejuni proteins expressed in vivo', Infection and immunity, vol. 60, no. 11, pp. 4938-4944.
Panigrahi P, Losonsky G, DeTolla LJ, Morris JG. Human immune response to Campylobacter jejuni proteins expressed in vivo. Infection and immunity. 1992 Jan 1;60(11):4938-4944.
Panigrahi, P. ; Losonsky, G. ; DeTolla, L. J. ; Morris, J. G. / Human immune response to Campylobacter jejuni proteins expressed in vivo. In: Infection and immunity. 1992 ; Vol. 60, No. 11. pp. 4938-4944.
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