Human endogenous retrovirus HC2 is a new member of the S71 retroviral subgroup with a full-length pol gene

Peter Kabát, Michael Tristem, Rene Opavsky, Jaromír Pastorek

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have isolated and characterized a new human endogenous provirus, which is closely related to the human retrovirus S71, but unlike S71 has a full-length pol gene. Two degenerate oligonucleotide primers based on highly conserved motifs within the active sites of two retroviral proteins (the protease and reverse transcriptase) were designed and used for PCR. An amplified product of 847 bp in length, which showed significant homology to protease and reverse transcriptase of several retroviruses, was used for high stringency hybridization with a human genomic library. The MuLV-related endogenous retrovirus sequence, designated HC2, was isolated and completely sequenced. HC2 is a provirus with complete gag and pol genes and a 3' LTR; the 5' LTR and env gene are missing. The gag and pol genes appear complete, since they contain sequences homologous to the matrix protein, capsid protein, and nucleocapsid protein of gag and to the protease, reverse transcriptase, tether, RNase H, and integrase of pol. Phylogenetic analysis suggests that although HC2 and S71 are MuLV-related retroviruses, their characters are quite distinct, being placed outside of a clade containing most of the previously characterized MuLV-related retroviruses such as GaLV, FeLV, BaEV, and SSV/SSAV.

Original languageEnglish (US)
Pages (from-to)83-94
Number of pages12
JournalVirology
Volume226
Issue number1
DOIs
StatePublished - Dec 1 1996

Fingerprint

pol Genes
Endogenous Retroviruses
Retroviridae
RNA-Directed DNA Polymerase
gag Genes
Proviruses
Peptide Hydrolases
Feline Leukemia Virus
Ribonuclease H
env Genes
Nucleocapsid Proteins
Integrases
DNA Primers
Genomic Library
Capsid Proteins
Sequence Homology
Catalytic Domain
Proteins
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology

Cite this

Human endogenous retrovirus HC2 is a new member of the S71 retroviral subgroup with a full-length pol gene. / Kabát, Peter; Tristem, Michael; Opavsky, Rene; Pastorek, Jaromír.

In: Virology, Vol. 226, No. 1, 01.12.1996, p. 83-94.

Research output: Contribution to journalArticle

Kabát, Peter ; Tristem, Michael ; Opavsky, Rene ; Pastorek, Jaromír. / Human endogenous retrovirus HC2 is a new member of the S71 retroviral subgroup with a full-length pol gene. In: Virology. 1996 ; Vol. 226, No. 1. pp. 83-94.
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