Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family

Andrew Koff, Fred Cross, Alfred Fisher, Jill Schumacher, Katherine Leguellec, Michel Philippe, James M. Roberts

Research output: Contribution to journalArticle

477 Citations (Scopus)

Abstract

A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells.

Original languageEnglish (US)
Pages (from-to)1217-1228
Number of pages12
JournalCell
Volume66
Issue number6
DOIs
StatePublished - Sep 20 1991

Fingerprint

Cyclin E
Cyclins
Genes
Xenopus
HeLa Cells
Yeast
Escherichia coli
Saccharomyces cerevisiae
Proteins
Yeasts
Cells
Mutation
Antibodies

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Koff, A., Cross, F., Fisher, A., Schumacher, J., Leguellec, K., Philippe, M., & Roberts, J. M. (1991). Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. Cell, 66(6), 1217-1228. https://doi.org/10.1016/0092-8674(91)90044-Y

Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. / Koff, Andrew; Cross, Fred; Fisher, Alfred; Schumacher, Jill; Leguellec, Katherine; Philippe, Michel; Roberts, James M.

In: Cell, Vol. 66, No. 6, 20.09.1991, p. 1217-1228.

Research output: Contribution to journalArticle

Koff, A, Cross, F, Fisher, A, Schumacher, J, Leguellec, K, Philippe, M & Roberts, JM 1991, 'Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family', Cell, vol. 66, no. 6, pp. 1217-1228. https://doi.org/10.1016/0092-8674(91)90044-Y
Koff A, Cross F, Fisher A, Schumacher J, Leguellec K, Philippe M et al. Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. Cell. 1991 Sep 20;66(6):1217-1228. https://doi.org/10.1016/0092-8674(91)90044-Y
Koff, Andrew ; Cross, Fred ; Fisher, Alfred ; Schumacher, Jill ; Leguellec, Katherine ; Philippe, Michel ; Roberts, James M. / Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family. In: Cell. 1991 ; Vol. 66, No. 6. pp. 1217-1228.
@article{8a16744c80094a0da24cc1976811458e,
title = "Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family",
abstract = "A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells.",
author = "Andrew Koff and Fred Cross and Alfred Fisher and Jill Schumacher and Katherine Leguellec and Michel Philippe and Roberts, {James M.}",
year = "1991",
month = "9",
day = "20",
doi = "10.1016/0092-8674(91)90044-Y",
language = "English (US)",
volume = "66",
pages = "1217--1228",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family

AU - Koff, Andrew

AU - Cross, Fred

AU - Fisher, Alfred

AU - Schumacher, Jill

AU - Leguellec, Katherine

AU - Philippe, Michel

AU - Roberts, James M.

PY - 1991/9/20

Y1 - 1991/9/20

N2 - A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells.

AB - A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells.

UR - http://www.scopus.com/inward/record.url?scp=0025734356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025734356&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(91)90044-Y

DO - 10.1016/0092-8674(91)90044-Y

M3 - Article

C2 - 1833068

AN - SCOPUS:0025734356

VL - 66

SP - 1217

EP - 1228

JO - Cell

JF - Cell

SN - 0092-8674

IS - 6

ER -