Human chorionic gonadotropin activates the inositol 1,4,5-trisphosphate-Ca2+ intracellular signalling system in bovine luteal cells

John S. Davis, Leigh A. West, Laura L. Weakland, Robert V. Farese

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Human chorionic gonadotropin, hCG, a hormone which increases intracellular cAMP, provoked rapid (30 s) and sustained (up to 30 min) increases in the levels of inositol mono-, bis- and trisphosphates (IP, IP2 and IP3, respectively) in bovine luteal cells. LiCl (10 mM) enhanced inositol phosphate accumulation in response to hCG. Concentration-dependent increases in inositol phosphates, cAMP and progesterone accumulation were observed in hCG-treated luteal cells. hCG also induced rapid and concentration-dependent increases in cytosolic free Ca2+ as measured by quin 2 fluorescence. These findings demonstrate that hCG stimulates the phospholipase C-IP3 and diacylglycerol 'second messenger' system in the bovine corpus luteum.

Original languageEnglish (US)
Pages (from-to)287-291
Number of pages5
JournalFEBS Letters
Volume208
Issue number2
DOIs
StatePublished - Nov 24 1986

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Luteal Cells
Inositol 1,4,5-Trisphosphate
Inositol Phosphates
Chorionic Gonadotropin
Corpus Luteum
Diglycerides
Type C Phospholipases
Second Messenger Systems
Inositol
Progesterone
Fluorescence
Hormones
Quin2

Keywords

  • Ca Gonadotropin Inositol phospholipid Inositol phosphate (Corpus luteum)

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Human chorionic gonadotropin activates the inositol 1,4,5-trisphosphate-Ca2+ intracellular signalling system in bovine luteal cells. / Davis, John S.; West, Leigh A.; Weakland, Laura L.; Farese, Robert V.

In: FEBS Letters, Vol. 208, No. 2, 24.11.1986, p. 287-291.

Research output: Contribution to journalArticle

Davis, John S. ; West, Leigh A. ; Weakland, Laura L. ; Farese, Robert V. / Human chorionic gonadotropin activates the inositol 1,4,5-trisphosphate-Ca2+ intracellular signalling system in bovine luteal cells. In: FEBS Letters. 1986 ; Vol. 208, No. 2. pp. 287-291.
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