Human chorionic gonadotropin-β gene expression in first trimester placenta

Amanda K. Miller-Lindholm, C. J. Labenz, Jacques Ramey, Elliott Bedows, Raymond W. Ruddon

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The hCGβ gene family contains six genes linked in tandem on chromosome 19 and labeled β genes 7, 8, 5, 1, 2, and 3. Previous studies on a small number of placentas have indicated that β gene 5 was the most highly expressed gene during the first trimester of pregnancy, followed by genes 3 and 8. β genes 7, 1, and 2 were expressed at very low levels. The purpose of this study was to determine 1) whether this pattern of expression was typical during normal pregnancy by sampling a large number of first trimester placentas, and 2) whether there was a correlation between gestational age and the pattern of hCGβ gene expression. Total RNA from 27 first trimester placentas varying in age from 6-16 weeks was reverse transcribed into complementary DNA. The complementary DNA was amplified by PCR, and the amount of DNA representative of each hCGβ gene was quantified by Genescan analysis. In 14 of the 27 placentas, hCGβ gene 5 accounted for 50% or more of the total β messenger RNA expressed. β gene 3 was expressed at levels ranging from 1-42% of the total, and β gene 8 expression ranged from 12-32% of the total. Gene 7 expression was less than 3% of the total β expression in all 27 placentas. Although there appeared to be a trend toward lower expression of β gene 3 in placentas beyond 10 weeks gestational age, there was no correlation of the pattern of β expression with placental age. β gene expression was also examined in two blighted ova a spontaneous abortion sample, and a hydatidiform mole as well as in cultured JAR choriocarcinoma cells. With the exception of JAR cells, these abnormal tissues had low levels of gene 3 expression, but these levels were within the range of the patterns observed in normal placentas. These data suggest that it is the total amount of hCGβ gene expression rather than the expression of individual β genes that is important for the maintenance of normal pregnancy.

Original languageEnglish (US)
Pages (from-to)5459-5465
Number of pages7
JournalEndocrinology
Volume138
Issue number12
DOIs
StatePublished - Jan 1 1997

Fingerprint

Chorionic Gonadotropin
First Pregnancy Trimester
Placenta
Gene Expression
Genes
Gestational Age
Complementary DNA
Pregnancy Maintenance
Chromosomes, Human, Pair 19
Hydatidiform Mole
Choriocarcinoma
Spontaneous Abortion
Ovum
RNA
Pregnancy
Polymerase Chain Reaction
Messenger RNA
DNA

ASJC Scopus subject areas

  • Endocrinology

Cite this

Miller-Lindholm, A. K., Labenz, C. J., Ramey, J., Bedows, E., & Ruddon, R. W. (1997). Human chorionic gonadotropin-β gene expression in first trimester placenta. Endocrinology, 138(12), 5459-5465. https://doi.org/10.1210/endo.138.12.5618

Human chorionic gonadotropin-β gene expression in first trimester placenta. / Miller-Lindholm, Amanda K.; Labenz, C. J.; Ramey, Jacques; Bedows, Elliott; Ruddon, Raymond W.

In: Endocrinology, Vol. 138, No. 12, 01.01.1997, p. 5459-5465.

Research output: Contribution to journalArticle

Miller-Lindholm, AK, Labenz, CJ, Ramey, J, Bedows, E & Ruddon, RW 1997, 'Human chorionic gonadotropin-β gene expression in first trimester placenta', Endocrinology, vol. 138, no. 12, pp. 5459-5465. https://doi.org/10.1210/endo.138.12.5618
Miller-Lindholm AK, Labenz CJ, Ramey J, Bedows E, Ruddon RW. Human chorionic gonadotropin-β gene expression in first trimester placenta. Endocrinology. 1997 Jan 1;138(12):5459-5465. https://doi.org/10.1210/endo.138.12.5618
Miller-Lindholm, Amanda K. ; Labenz, C. J. ; Ramey, Jacques ; Bedows, Elliott ; Ruddon, Raymond W. / Human chorionic gonadotropin-β gene expression in first trimester placenta. In: Endocrinology. 1997 ; Vol. 138, No. 12. pp. 5459-5465.
@article{d500491777764a0888973b1137a326eb,
title = "Human chorionic gonadotropin-β gene expression in first trimester placenta",
abstract = "The hCGβ gene family contains six genes linked in tandem on chromosome 19 and labeled β genes 7, 8, 5, 1, 2, and 3. Previous studies on a small number of placentas have indicated that β gene 5 was the most highly expressed gene during the first trimester of pregnancy, followed by genes 3 and 8. β genes 7, 1, and 2 were expressed at very low levels. The purpose of this study was to determine 1) whether this pattern of expression was typical during normal pregnancy by sampling a large number of first trimester placentas, and 2) whether there was a correlation between gestational age and the pattern of hCGβ gene expression. Total RNA from 27 first trimester placentas varying in age from 6-16 weeks was reverse transcribed into complementary DNA. The complementary DNA was amplified by PCR, and the amount of DNA representative of each hCGβ gene was quantified by Genescan analysis. In 14 of the 27 placentas, hCGβ gene 5 accounted for 50{\%} or more of the total β messenger RNA expressed. β gene 3 was expressed at levels ranging from 1-42{\%} of the total, and β gene 8 expression ranged from 12-32{\%} of the total. Gene 7 expression was less than 3{\%} of the total β expression in all 27 placentas. Although there appeared to be a trend toward lower expression of β gene 3 in placentas beyond 10 weeks gestational age, there was no correlation of the pattern of β expression with placental age. β gene expression was also examined in two blighted ova a spontaneous abortion sample, and a hydatidiform mole as well as in cultured JAR choriocarcinoma cells. With the exception of JAR cells, these abnormal tissues had low levels of gene 3 expression, but these levels were within the range of the patterns observed in normal placentas. These data suggest that it is the total amount of hCGβ gene expression rather than the expression of individual β genes that is important for the maintenance of normal pregnancy.",
author = "Miller-Lindholm, {Amanda K.} and Labenz, {C. J.} and Jacques Ramey and Elliott Bedows and Ruddon, {Raymond W.}",
year = "1997",
month = "1",
day = "1",
doi = "10.1210/endo.138.12.5618",
language = "English (US)",
volume = "138",
pages = "5459--5465",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "12",

}

TY - JOUR

T1 - Human chorionic gonadotropin-β gene expression in first trimester placenta

AU - Miller-Lindholm, Amanda K.

AU - Labenz, C. J.

AU - Ramey, Jacques

AU - Bedows, Elliott

AU - Ruddon, Raymond W.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The hCGβ gene family contains six genes linked in tandem on chromosome 19 and labeled β genes 7, 8, 5, 1, 2, and 3. Previous studies on a small number of placentas have indicated that β gene 5 was the most highly expressed gene during the first trimester of pregnancy, followed by genes 3 and 8. β genes 7, 1, and 2 were expressed at very low levels. The purpose of this study was to determine 1) whether this pattern of expression was typical during normal pregnancy by sampling a large number of first trimester placentas, and 2) whether there was a correlation between gestational age and the pattern of hCGβ gene expression. Total RNA from 27 first trimester placentas varying in age from 6-16 weeks was reverse transcribed into complementary DNA. The complementary DNA was amplified by PCR, and the amount of DNA representative of each hCGβ gene was quantified by Genescan analysis. In 14 of the 27 placentas, hCGβ gene 5 accounted for 50% or more of the total β messenger RNA expressed. β gene 3 was expressed at levels ranging from 1-42% of the total, and β gene 8 expression ranged from 12-32% of the total. Gene 7 expression was less than 3% of the total β expression in all 27 placentas. Although there appeared to be a trend toward lower expression of β gene 3 in placentas beyond 10 weeks gestational age, there was no correlation of the pattern of β expression with placental age. β gene expression was also examined in two blighted ova a spontaneous abortion sample, and a hydatidiform mole as well as in cultured JAR choriocarcinoma cells. With the exception of JAR cells, these abnormal tissues had low levels of gene 3 expression, but these levels were within the range of the patterns observed in normal placentas. These data suggest that it is the total amount of hCGβ gene expression rather than the expression of individual β genes that is important for the maintenance of normal pregnancy.

AB - The hCGβ gene family contains six genes linked in tandem on chromosome 19 and labeled β genes 7, 8, 5, 1, 2, and 3. Previous studies on a small number of placentas have indicated that β gene 5 was the most highly expressed gene during the first trimester of pregnancy, followed by genes 3 and 8. β genes 7, 1, and 2 were expressed at very low levels. The purpose of this study was to determine 1) whether this pattern of expression was typical during normal pregnancy by sampling a large number of first trimester placentas, and 2) whether there was a correlation between gestational age and the pattern of hCGβ gene expression. Total RNA from 27 first trimester placentas varying in age from 6-16 weeks was reverse transcribed into complementary DNA. The complementary DNA was amplified by PCR, and the amount of DNA representative of each hCGβ gene was quantified by Genescan analysis. In 14 of the 27 placentas, hCGβ gene 5 accounted for 50% or more of the total β messenger RNA expressed. β gene 3 was expressed at levels ranging from 1-42% of the total, and β gene 8 expression ranged from 12-32% of the total. Gene 7 expression was less than 3% of the total β expression in all 27 placentas. Although there appeared to be a trend toward lower expression of β gene 3 in placentas beyond 10 weeks gestational age, there was no correlation of the pattern of β expression with placental age. β gene expression was also examined in two blighted ova a spontaneous abortion sample, and a hydatidiform mole as well as in cultured JAR choriocarcinoma cells. With the exception of JAR cells, these abnormal tissues had low levels of gene 3 expression, but these levels were within the range of the patterns observed in normal placentas. These data suggest that it is the total amount of hCGβ gene expression rather than the expression of individual β genes that is important for the maintenance of normal pregnancy.

UR - http://www.scopus.com/inward/record.url?scp=0030670914&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030670914&partnerID=8YFLogxK

U2 - 10.1210/endo.138.12.5618

DO - 10.1210/endo.138.12.5618

M3 - Article

C2 - 9389532

AN - SCOPUS:0030670914

VL - 138

SP - 5459

EP - 5465

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 12

ER -