Human butyrylcholinesterase produced in insect cells: Huprine-based affinity purification and crystal structure

Xavier Brazzolotto, Marielle Wandhammer, Cyril Ronco, Marie Trovaslet, Ludovic Jean, Oksana Lockridge, Pierre Yves Renard, Florian Nachon

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Butyrylcholinesterase (BChE) is a serine hydrolase that is present in all mammalian tissues. It can accommodate larger substrates or inhibitors than acetylcholinesterase (AChE), the enzyme responsible for hydrolysis of the neurotransmitter acetylcholine in the central nervous system and neuromuscular junctions. AChE is the specific target of organophosphorous pesticides and warfare nerve agents, and BChE is a stoichiometric bioscavenger. Conversion of BChE into a catalytic bioscavenger by rational design or designing reactivators specific to BChE required structural data obtained using a recombinant low-glycosylated human BChE expressed in Chinese hamster ovary cells. This expression system yields ∼ 1 mg of pure enzyme per litre of cell culture. Here, we report an improved expression system using insect cells with a fourfold higher yield for truncated human BChE with all glycosylation sites present. We developed a fast purification protocol for the recombinant protein using huprine-based affinity chromatography, which is superior to the classical procainamide-based affinity. The purified BChE crystallized under different conditions and space group than the recombinant low-glycosylated protein produced in Chinese hamster ovary cells. The crystals diffracted to 2.5 Å The overall monomer structure is similar to the low-glycosylated structure except for the presence of the additional glycans. Remarkably, the carboxylic acid molecule systematically bound to the catalytic serine in the low-glycosylated structure is also present in this new structure, despite the different expression system, purification protocol and crystallization conditions.

Original languageEnglish (US)
Pages (from-to)2905-2916
Number of pages12
JournalFEBS Journal
Volume279
Issue number16
DOIs
StatePublished - Aug 1 2012

Fingerprint

Butyrylcholinesterase
Purification
Insects
Crystal structure
Cricetulus
Serine
Ovary
Cells
Glycosylation
Affinity chromatography
Procainamide
Neuromuscular Junction
Military operations
Cholinesterase Inhibitors
Neurology
Enzyme Inhibitors
Hydrolases
Enzymes
Acetylcholinesterase
Carboxylic Acids

Keywords

  • X-ray structure
  • affinity chromatography
  • butyrylcholinesterase
  • huprine
  • insect cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Human butyrylcholinesterase produced in insect cells : Huprine-based affinity purification and crystal structure. / Brazzolotto, Xavier; Wandhammer, Marielle; Ronco, Cyril; Trovaslet, Marie; Jean, Ludovic; Lockridge, Oksana; Renard, Pierre Yves; Nachon, Florian.

In: FEBS Journal, Vol. 279, No. 16, 01.08.2012, p. 2905-2916.

Research output: Contribution to journalArticle

Brazzolotto, X, Wandhammer, M, Ronco, C, Trovaslet, M, Jean, L, Lockridge, O, Renard, PY & Nachon, F 2012, 'Human butyrylcholinesterase produced in insect cells: Huprine-based affinity purification and crystal structure', FEBS Journal, vol. 279, no. 16, pp. 2905-2916. https://doi.org/10.1111/j.1742-4658.2012.08672.x
Brazzolotto, Xavier ; Wandhammer, Marielle ; Ronco, Cyril ; Trovaslet, Marie ; Jean, Ludovic ; Lockridge, Oksana ; Renard, Pierre Yves ; Nachon, Florian. / Human butyrylcholinesterase produced in insect cells : Huprine-based affinity purification and crystal structure. In: FEBS Journal. 2012 ; Vol. 279, No. 16. pp. 2905-2916.
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