Human bronchial epithelial cells modulate collagen gel contraction by fibroblasts

Tadashi Mio, Xiang-de Liu, Yuichi Adachi, Ilja Striz, C. Magnus Sköld, Debra Romberger, John R. Spurzem, Mary G. Illig, Ron Ertl, Stephen I. Rennard

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Connective tissue contraction is an important aspect of both normal wound healing and fibrosis. This process may contribute to small airway narrowing associated with certain airway diseases. Fibroblast-mediated contraction of a three-dimensional collagen gel has been considered a model of tissue contraction. In this study, the ability of primary cultured human bronchial epithelial cells (HBEC) obtained by bronchial brushings to modulate fibroblast gel contraction was evaluated. Human lung fibroblasts (HFL1) were cast into type I collagen gels. The gels were floated both in dishes containing a monolayer of HBEC or in dishes without HBEC. Contraction assessed by measuring the area of gels was increased at all time points from 24 h up to 96 h of coculture. At 48 h, coculture of HBEC with fibroblasts resulted in significantly more contraction than fibroblasts alone (36.6 ± 1.2 vs. 20.4 ± 1.7%, P < 0.05). Lipopolysaccharide (LPS, 10 μg/ml) stimulation of the HBEC augmented the contraction (44.9 ± 1.0%, P < 0.05 vs. HBEC). In the presence of indomethacin, the augmentation by LPS was increased further (52.2 ± 4.3%, P < 0.05 vs. HBEC with LPS), suggesting that prostaglandins (PGs) are present and may inhibit contraction. Consistent with this, PGE was present in HBEC-conditioned medium. Bronchial epithelial cell conditioned medium had an effect similar to coculturing. SG-150 column chromatography revealed augmentive activity between 20 and 30 kDa and inhibitory activity between 10 and 20 kDa. Measurement by enzyme-linked immunosorbent assay confirmed the presence of the active form of transforming growth factor (TGF)-β2. The stimulatory activity of conditioned medium was blocked by adding anti-TGF-β antibody. These data demonstrate that, through the release of factors including TGF-β2 which can augment and PGE which can inhibit, HBEC can modulate fibroblast-mediated collagen gel contraction. In this manner, HBEC may modulate fibroblast activities that determine the architecture of bronchial tissue.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume274
Issue number1 18-1
StatePublished - Jan 1 1998

Fingerprint

Collagen
Fibroblasts
Gels
Epithelial Cells
Transforming Growth Factors
Conditioned Culture Medium
Coculture Techniques
Prostaglandins E
Collagen Type I
Indomethacin
Connective Tissue
Wound Healing
Prostaglandins
Lipopolysaccharides
Chromatography
Fibrosis
Enzyme-Linked Immunosorbent Assay
Lung
Antibodies

Keywords

  • Remodeling
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Human bronchial epithelial cells modulate collagen gel contraction by fibroblasts. / Mio, Tadashi; Liu, Xiang-de; Adachi, Yuichi; Striz, Ilja; Sköld, C. Magnus; Romberger, Debra; Spurzem, John R.; Illig, Mary G.; Ertl, Ron; Rennard, Stephen I.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 274, No. 1 18-1, 01.01.1998.

Research output: Contribution to journalArticle

Mio, T, Liu, X, Adachi, Y, Striz, I, Sköld, CM, Romberger, D, Spurzem, JR, Illig, MG, Ertl, R & Rennard, SI 1998, 'Human bronchial epithelial cells modulate collagen gel contraction by fibroblasts', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 274, no. 1 18-1.
Mio, Tadashi ; Liu, Xiang-de ; Adachi, Yuichi ; Striz, Ilja ; Sköld, C. Magnus ; Romberger, Debra ; Spurzem, John R. ; Illig, Mary G. ; Ertl, Ron ; Rennard, Stephen I. / Human bronchial epithelial cells modulate collagen gel contraction by fibroblasts. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1998 ; Vol. 274, No. 1 18-1.
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abstract = "Connective tissue contraction is an important aspect of both normal wound healing and fibrosis. This process may contribute to small airway narrowing associated with certain airway diseases. Fibroblast-mediated contraction of a three-dimensional collagen gel has been considered a model of tissue contraction. In this study, the ability of primary cultured human bronchial epithelial cells (HBEC) obtained by bronchial brushings to modulate fibroblast gel contraction was evaluated. Human lung fibroblasts (HFL1) were cast into type I collagen gels. The gels were floated both in dishes containing a monolayer of HBEC or in dishes without HBEC. Contraction assessed by measuring the area of gels was increased at all time points from 24 h up to 96 h of coculture. At 48 h, coculture of HBEC with fibroblasts resulted in significantly more contraction than fibroblasts alone (36.6 ± 1.2 vs. 20.4 ± 1.7{\%}, P < 0.05). Lipopolysaccharide (LPS, 10 μg/ml) stimulation of the HBEC augmented the contraction (44.9 ± 1.0{\%}, P < 0.05 vs. HBEC). In the presence of indomethacin, the augmentation by LPS was increased further (52.2 ± 4.3{\%}, P < 0.05 vs. HBEC with LPS), suggesting that prostaglandins (PGs) are present and may inhibit contraction. Consistent with this, PGE was present in HBEC-conditioned medium. Bronchial epithelial cell conditioned medium had an effect similar to coculturing. SG-150 column chromatography revealed augmentive activity between 20 and 30 kDa and inhibitory activity between 10 and 20 kDa. Measurement by enzyme-linked immunosorbent assay confirmed the presence of the active form of transforming growth factor (TGF)-β2. The stimulatory activity of conditioned medium was blocked by adding anti-TGF-β antibody. These data demonstrate that, through the release of factors including TGF-β2 which can augment and PGE which can inhibit, HBEC can modulate fibroblast-mediated collagen gel contraction. In this manner, HBEC may modulate fibroblast activities that determine the architecture of bronchial tissue.",
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AU - Romberger, Debra

AU - Spurzem, John R.

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AU - Ertl, Ron

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