Htlv-i sequence in lymphoproliferative disorders

W. C. Chan, C. Hooper, R. Wickert, J. M. Benson, J. Vardiman, Steven Heye Hinrichs, D. Weisenburger

Research output: Contribution to journalArticle

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Abstract

Several recent studies reported the detection of partially deleted HTLV-I provirus in biopsies of lesions from patients with mycosis fungoides (MF) and T-cell anaplastic large-cell lymphoma. We studied lesions from 59 patients (21 B-cell lymphomas: 16 diffuse and five follicular; 11 cutaneous T-cell lymphomas, including seven MF; one T-immunoblastic lymphoma; 10 diffuse anaplastic large-cell lymphomas: two B, four T, and four of indeterminate phenotype; three Hodgkin s lymphomas; eight atypical lymphoid proliferations; four other lymphoid lesions, and one squamous-cell carcinoma) using primers to the gag, pol and pX regions of HTLV-I in the polymerase chain reaction (PCR) to detect relevant sequences. A total of 10 patients showed one or more PCR-amplifiable products, including five of 11 patients with cutaneous T-cell lymphomas (45%) as compared with one of 21 patients with B-cell lymphomas (4.3%). We did not find a high incidence of positivity in anaplastic large-cell lymphomas, as reported previously. Detectable HTLV-I sequences were not limited to any subtype of lymphoma, and a pX sequence was detected in a squamous-cell carcinoma. Sequence analysis of one amplified product from each of the three regions studied showed a 94.2, 100, and 98.9% homology to the corresponding prototypical gag, pol, and pX HTLV-I sequences, respectively, indicating that the amplified sequences were derived from HTLV-I or a very closely related virus. HTLV-I sequences were detected in a significant proportion of patients with cutaneous T-cell lymphoma, but their role in the pathogenesis of the neoplasm is still unclear.

Original languageEnglish (US)
Pages (from-to)192-199
Number of pages8
JournalDiagnostic Molecular Pathology
Volume2
Issue number1
DOIs
StatePublished - Jan 1 1993

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Human T-lymphotropic virus 1
Lymphoproliferative Disorders
Anaplastic Large-Cell Lymphoma
Cutaneous T-Cell Lymphoma
Mycosis Fungoides
B-Cell Lymphoma
Squamous Cell Carcinoma
Lymphoma
Proviruses
Polymerase Chain Reaction
Lymphoma, Large B-Cell, Diffuse
T-Cell Lymphoma
Hodgkin Disease
Sequence Analysis
Viruses
Phenotype
Biopsy
Incidence
Neoplasms

Keywords

  • Cutaneous T-cell lymphoma
  • HTLV-I
  • Ki-1 lymphoma
  • Large-cell anaplastic lymphoma
  • Mycosis fungoides/Sezary syndrome
  • Retroviruses

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Chan, W. C., Hooper, C., Wickert, R., Benson, J. M., Vardiman, J., Hinrichs, S. H., & Weisenburger, D. (1993). Htlv-i sequence in lymphoproliferative disorders. Diagnostic Molecular Pathology, 2(1), 192-199. https://doi.org/10.1097/00019606-199309000-00007

Htlv-i sequence in lymphoproliferative disorders. / Chan, W. C.; Hooper, C.; Wickert, R.; Benson, J. M.; Vardiman, J.; Hinrichs, Steven Heye; Weisenburger, D.

In: Diagnostic Molecular Pathology, Vol. 2, No. 1, 01.01.1993, p. 192-199.

Research output: Contribution to journalArticle

Chan, WC, Hooper, C, Wickert, R, Benson, JM, Vardiman, J, Hinrichs, SH & Weisenburger, D 1993, 'Htlv-i sequence in lymphoproliferative disorders', Diagnostic Molecular Pathology, vol. 2, no. 1, pp. 192-199. https://doi.org/10.1097/00019606-199309000-00007
Chan, W. C. ; Hooper, C. ; Wickert, R. ; Benson, J. M. ; Vardiman, J. ; Hinrichs, Steven Heye ; Weisenburger, D. / Htlv-i sequence in lymphoproliferative disorders. In: Diagnostic Molecular Pathology. 1993 ; Vol. 2, No. 1. pp. 192-199.
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abstract = "Several recent studies reported the detection of partially deleted HTLV-I provirus in biopsies of lesions from patients with mycosis fungoides (MF) and T-cell anaplastic large-cell lymphoma. We studied lesions from 59 patients (21 B-cell lymphomas: 16 diffuse and five follicular; 11 cutaneous T-cell lymphomas, including seven MF; one T-immunoblastic lymphoma; 10 diffuse anaplastic large-cell lymphomas: two B, four T, and four of indeterminate phenotype; three Hodgkin s lymphomas; eight atypical lymphoid proliferations; four other lymphoid lesions, and one squamous-cell carcinoma) using primers to the gag, pol and pX regions of HTLV-I in the polymerase chain reaction (PCR) to detect relevant sequences. A total of 10 patients showed one or more PCR-amplifiable products, including five of 11 patients with cutaneous T-cell lymphomas (45{\%}) as compared with one of 21 patients with B-cell lymphomas (4.3{\%}). We did not find a high incidence of positivity in anaplastic large-cell lymphomas, as reported previously. Detectable HTLV-I sequences were not limited to any subtype of lymphoma, and a pX sequence was detected in a squamous-cell carcinoma. Sequence analysis of one amplified product from each of the three regions studied showed a 94.2, 100, and 98.9{\%} homology to the corresponding prototypical gag, pol, and pX HTLV-I sequences, respectively, indicating that the amplified sequences were derived from HTLV-I or a very closely related virus. HTLV-I sequences were detected in a significant proportion of patients with cutaneous T-cell lymphoma, but their role in the pathogenesis of the neoplasm is still unclear.",
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