hTERT can function with rabbit telomerase RNA

Regulation of gene expression and attenuation of apoptosis

Hua Xiang, Juan Wang, Ying Wei Mao, David W Li

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Telomerase is a specialized DNA polymerase that adds telomeric sequences onto chromosome ends. The functional telomerase complex contains a telomerase reverse transcriptase (TERT) and also a telomerase RNA (TR). Although it is well established that the human telomerase reverse transcriptase (hTERT) can function well in different human cell lines, it has not been shown whether it is compatible with telomerase template RNA from other species. Here we report that the expressed hTERT is functionally compatible with rabbit telomerase template RNA (rTR) as demonstrated by TRAP assay. The direct interaction between hTERT and rTR is further confirmed by immunoprecipitation-linked RT-PCR in which rTR is detected from the complex immunoprecipitated by an anti-hTERT antibody. The hTERT expressed in rabbit lens epithelial cells demonstrates two major functions: modulation of expression of other genes and attenuation of apoptosis. Thus, telomerase has a variety of functions besides telomere synthesis, and the template RNA is functionally conserved between human and rabbit. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)503-510
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume278
Issue number3
DOIs
StatePublished - Nov 30 2000

Fingerprint

Telomerase
Gene Expression Regulation
Gene expression
Apoptosis
Rabbits
RNA
Telomere
DNA-Directed DNA Polymerase
Immunoprecipitation
Lenses
Chromosomes
Epithelial Cells
human TERT protein
telomerase RNA
Gene Expression
Cell Line
Polymerase Chain Reaction
Antibodies
Assays
Genes

Keywords

  • AP-1
  • Apoptosis
  • Caspase-3
  • Human telomerase reverse transcriptase (hTERT)
  • Rabbit lens epithelial cells
  • Telomerase RNA (TR)
  • bcl-xS
  • c-fos
  • c-jun
  • p53

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

hTERT can function with rabbit telomerase RNA : Regulation of gene expression and attenuation of apoptosis. / Xiang, Hua; Wang, Juan; Mao, Ying Wei; Li, David W.

In: Biochemical and Biophysical Research Communications, Vol. 278, No. 3, 30.11.2000, p. 503-510.

Research output: Contribution to journalArticle

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