HRT gene function requires interaction between a NAC protein and viral capsid protein to confer resistance to turnip crinkle virus

T. Ren, F. Qu, T. J. Morris

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

An Arabidopsis protein was found to interact specifically with the capsid protein (CP) of turnip crinkle virus (TCV) through yeast two-hybrid screening. This protein, designated TIP (for TCV-interacting protein), was found to be a member of the recently recognized NAC family of proteins. NAC proteins have been implicated in the regulation of development of plant embryos and flowers. TIP alone was able to activate expression of reporter genes in yeast if fused to a DNA binding domain, suggesting that it may be a transcriptional activator. The TIP binding region in the TCV CP has been mapped to the N-terminal 25 amino acids. Site-directed mutagenesis within this region revealed that loss of the TIP-CP interaction in the yeast two-hybrid assay correlated with loss of the ability of TCV to induce the hypersensitive response and resistance in the TCV-resistant Arabidopsis ecotype Dijon (Di-0 and its inbred line Di-17). These data suggest that TIP is an essential component in the TCV resistance response pathway.

Original languageEnglish (US)
Pages (from-to)1917-1925
Number of pages9
JournalPlant Cell
Volume12
Issue number10
DOIs
StatePublished - Nov 22 2000

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Carmovirus
Turnip crinkle virus
viral proteins
Capsid Proteins
Viral Proteins
coat proteins
Viruses
Genes
Yeast
Proteins
genes
proteins
two hybrid system techniques
Arabidopsis Proteins
Yeasts
Mutagenesis
Ecotype
Arabidopsis
Two-Hybrid System Techniques
yeasts

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

HRT gene function requires interaction between a NAC protein and viral capsid protein to confer resistance to turnip crinkle virus. / Ren, T.; Qu, F.; Morris, T. J.

In: Plant Cell, Vol. 12, No. 10, 22.11.2000, p. 1917-1925.

Research output: Contribution to journalArticle

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