HPV types and variants among cervical cancer tumors in three regions of Tunisia

Keris KrennHrubec, Karima Mrad, Badreddine Sriha, Farhat Ben Ayed, Danielle M. Bottalico, Janae Ostolaza, Benjamin Smith, Tatyana Tchaikovska, Amr S Soliman, Robert D. Burk

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cervical cancer is the second most common cancer among Tunisian women, and the incidence rates vary by region. Three Tunisian registries report age-standardized rates of 6.3/105 in the central region, 5.4/105 in the north, and 2.7/105 in the south. High-risk human papillomavirus (HPV) types and their variants differ in carcinogenic potential and geographic distribution. The HPV type and variant distribution could be a factor in the differing rates between regions of Tunisia. Tumor tissue was collected from 142 Tunisian cervical cancer patients. Demographic and reproductive characteristics of the patients were abstracted from cancer registry and hospital records. HPV type and variant analyses were performed using PCR-based Luminex and dot-blot hybridization assays. Eighty-three percent of tumors were infected with at least one HPV type. European variants of HPV16/18 were the most prevalent in tumors from all three regions, with all HPV18 infections and 64% of HPV16 infections being of European lineage. A higher frequency of HPV16 was present in Northern Tunisia (80%) than in Central (68%) or Southern Tunisia (50%) (P=0.02). HPV18/45 was significantly more common in adenocarcinomas (50%) than in squamous cell carcinomas (11%) (P=0.004). Frequent infection with European HPV variants most likely reflects the history of European migration to Tunisia. In addition to the importance of understanding the variants of HPV in Tunisia, behavioral and cultural attitudes towards screening and age-specific infection rates should be investigated to aid the development of future vaccination and HPV screening programs and policies.

Original languageEnglish (US)
Pages (from-to)651-657
Number of pages7
JournalJournal of Medical Virology
Volume83
Issue number4
DOIs
StatePublished - Apr 1 2011

Fingerprint

Tunisia
Uterine Cervical Neoplasms
Neoplasms
Infection
Registries
Cancer Care Facilities
Hospital Records
Squamous Cell Carcinoma
Vaccination
Adenocarcinoma
History
Demography
Polymerase Chain Reaction
Incidence

Keywords

  • Cancer Registry
  • HPV variants
  • HPV16
  • Human papillomavirus (HPV)
  • North Africa

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

KrennHrubec, K., Mrad, K., Sriha, B., Ayed, F. B., Bottalico, D. M., Ostolaza, J., ... Burk, R. D. (2011). HPV types and variants among cervical cancer tumors in three regions of Tunisia. Journal of Medical Virology, 83(4), 651-657. https://doi.org/10.1002/jmv.22011

HPV types and variants among cervical cancer tumors in three regions of Tunisia. / KrennHrubec, Keris; Mrad, Karima; Sriha, Badreddine; Ayed, Farhat Ben; Bottalico, Danielle M.; Ostolaza, Janae; Smith, Benjamin; Tchaikovska, Tatyana; Soliman, Amr S; Burk, Robert D.

In: Journal of Medical Virology, Vol. 83, No. 4, 01.04.2011, p. 651-657.

Research output: Contribution to journalArticle

KrennHrubec, K, Mrad, K, Sriha, B, Ayed, FB, Bottalico, DM, Ostolaza, J, Smith, B, Tchaikovska, T, Soliman, AS & Burk, RD 2011, 'HPV types and variants among cervical cancer tumors in three regions of Tunisia', Journal of Medical Virology, vol. 83, no. 4, pp. 651-657. https://doi.org/10.1002/jmv.22011
KrennHrubec K, Mrad K, Sriha B, Ayed FB, Bottalico DM, Ostolaza J et al. HPV types and variants among cervical cancer tumors in three regions of Tunisia. Journal of Medical Virology. 2011 Apr 1;83(4):651-657. https://doi.org/10.1002/jmv.22011
KrennHrubec, Keris ; Mrad, Karima ; Sriha, Badreddine ; Ayed, Farhat Ben ; Bottalico, Danielle M. ; Ostolaza, Janae ; Smith, Benjamin ; Tchaikovska, Tatyana ; Soliman, Amr S ; Burk, Robert D. / HPV types and variants among cervical cancer tumors in three regions of Tunisia. In: Journal of Medical Virology. 2011 ; Vol. 83, No. 4. pp. 651-657.
@article{c13318f8261e489c8e21b1c119b4d41e,
title = "HPV types and variants among cervical cancer tumors in three regions of Tunisia",
abstract = "Cervical cancer is the second most common cancer among Tunisian women, and the incidence rates vary by region. Three Tunisian registries report age-standardized rates of 6.3/105 in the central region, 5.4/105 in the north, and 2.7/105 in the south. High-risk human papillomavirus (HPV) types and their variants differ in carcinogenic potential and geographic distribution. The HPV type and variant distribution could be a factor in the differing rates between regions of Tunisia. Tumor tissue was collected from 142 Tunisian cervical cancer patients. Demographic and reproductive characteristics of the patients were abstracted from cancer registry and hospital records. HPV type and variant analyses were performed using PCR-based Luminex and dot-blot hybridization assays. Eighty-three percent of tumors were infected with at least one HPV type. European variants of HPV16/18 were the most prevalent in tumors from all three regions, with all HPV18 infections and 64{\%} of HPV16 infections being of European lineage. A higher frequency of HPV16 was present in Northern Tunisia (80{\%}) than in Central (68{\%}) or Southern Tunisia (50{\%}) (P=0.02). HPV18/45 was significantly more common in adenocarcinomas (50{\%}) than in squamous cell carcinomas (11{\%}) (P=0.004). Frequent infection with European HPV variants most likely reflects the history of European migration to Tunisia. In addition to the importance of understanding the variants of HPV in Tunisia, behavioral and cultural attitudes towards screening and age-specific infection rates should be investigated to aid the development of future vaccination and HPV screening programs and policies.",
keywords = "Cancer Registry, HPV variants, HPV16, Human papillomavirus (HPV), North Africa",
author = "Keris KrennHrubec and Karima Mrad and Badreddine Sriha and Ayed, {Farhat Ben} and Bottalico, {Danielle M.} and Janae Ostolaza and Benjamin Smith and Tatyana Tchaikovska and Soliman, {Amr S} and Burk, {Robert D.}",
year = "2011",
month = "4",
day = "1",
doi = "10.1002/jmv.22011",
language = "English (US)",
volume = "83",
pages = "651--657",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - HPV types and variants among cervical cancer tumors in three regions of Tunisia

AU - KrennHrubec, Keris

AU - Mrad, Karima

AU - Sriha, Badreddine

AU - Ayed, Farhat Ben

AU - Bottalico, Danielle M.

AU - Ostolaza, Janae

AU - Smith, Benjamin

AU - Tchaikovska, Tatyana

AU - Soliman, Amr S

AU - Burk, Robert D.

PY - 2011/4/1

Y1 - 2011/4/1

N2 - Cervical cancer is the second most common cancer among Tunisian women, and the incidence rates vary by region. Three Tunisian registries report age-standardized rates of 6.3/105 in the central region, 5.4/105 in the north, and 2.7/105 in the south. High-risk human papillomavirus (HPV) types and their variants differ in carcinogenic potential and geographic distribution. The HPV type and variant distribution could be a factor in the differing rates between regions of Tunisia. Tumor tissue was collected from 142 Tunisian cervical cancer patients. Demographic and reproductive characteristics of the patients were abstracted from cancer registry and hospital records. HPV type and variant analyses were performed using PCR-based Luminex and dot-blot hybridization assays. Eighty-three percent of tumors were infected with at least one HPV type. European variants of HPV16/18 were the most prevalent in tumors from all three regions, with all HPV18 infections and 64% of HPV16 infections being of European lineage. A higher frequency of HPV16 was present in Northern Tunisia (80%) than in Central (68%) or Southern Tunisia (50%) (P=0.02). HPV18/45 was significantly more common in adenocarcinomas (50%) than in squamous cell carcinomas (11%) (P=0.004). Frequent infection with European HPV variants most likely reflects the history of European migration to Tunisia. In addition to the importance of understanding the variants of HPV in Tunisia, behavioral and cultural attitudes towards screening and age-specific infection rates should be investigated to aid the development of future vaccination and HPV screening programs and policies.

AB - Cervical cancer is the second most common cancer among Tunisian women, and the incidence rates vary by region. Three Tunisian registries report age-standardized rates of 6.3/105 in the central region, 5.4/105 in the north, and 2.7/105 in the south. High-risk human papillomavirus (HPV) types and their variants differ in carcinogenic potential and geographic distribution. The HPV type and variant distribution could be a factor in the differing rates between regions of Tunisia. Tumor tissue was collected from 142 Tunisian cervical cancer patients. Demographic and reproductive characteristics of the patients were abstracted from cancer registry and hospital records. HPV type and variant analyses were performed using PCR-based Luminex and dot-blot hybridization assays. Eighty-three percent of tumors were infected with at least one HPV type. European variants of HPV16/18 were the most prevalent in tumors from all three regions, with all HPV18 infections and 64% of HPV16 infections being of European lineage. A higher frequency of HPV16 was present in Northern Tunisia (80%) than in Central (68%) or Southern Tunisia (50%) (P=0.02). HPV18/45 was significantly more common in adenocarcinomas (50%) than in squamous cell carcinomas (11%) (P=0.004). Frequent infection with European HPV variants most likely reflects the history of European migration to Tunisia. In addition to the importance of understanding the variants of HPV in Tunisia, behavioral and cultural attitudes towards screening and age-specific infection rates should be investigated to aid the development of future vaccination and HPV screening programs and policies.

KW - Cancer Registry

KW - HPV variants

KW - HPV16

KW - Human papillomavirus (HPV)

KW - North Africa

UR - http://www.scopus.com/inward/record.url?scp=79951528211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951528211&partnerID=8YFLogxK

U2 - 10.1002/jmv.22011

DO - 10.1002/jmv.22011

M3 - Article

VL - 83

SP - 651

EP - 657

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 4

ER -