How to use dynamic light scattering to improve the likelihood of growing macromolecular crystals

Research output: Chapter in Book/Report/Conference proceedingChapter

24 Citations (Scopus)

Abstract

Dynamic light scattering (DLS) has become one of the most useful diagnostic tools for crystallization. The main purpose of using DLS in crystal screening is to help the investigator understand the size distribution, stability, and aggregation state of macromolecules in solution. It can also be used to understand how experimental variables influence aggregation. With commercially available instruments, DLS is easy to perform, and most of the sample is recoverable. Most usefully, the homogeneity or monodispersity of a sample, as measured by DLS, can be predictive of crystallizability.

Original languageEnglish (US)
Title of host publicationMacromolecular Crystallography Protocols, Volume 1
Subtitle of host publicationPreparation and Crystallizationof Macromolecules
EditorsSylvie Doublie
Pages109-129
Number of pages21
DOIs
StatePublished - Feb 5 2007
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume363
ISSN (Print)1064-3745

Fingerprint

Crystallization
Research Personnel
Dynamic Light Scattering

Keywords

  • Aggregation
  • DLS
  • Dynamic light scattering
  • Hydrodynamic radius
  • Monodispersity
  • Polydispersity
  • Solubility

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Borgstahl, G. E. O. (2007). How to use dynamic light scattering to improve the likelihood of growing macromolecular crystals. In S. Doublie (Ed.), Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallizationof Macromolecules (pp. 109-129). (Methods in Molecular Biology; Vol. 363). https://doi.org/10.1385/1-59745-209-2:109

How to use dynamic light scattering to improve the likelihood of growing macromolecular crystals. / Borgstahl, Gloria E.O.

Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallizationof Macromolecules. ed. / Sylvie Doublie. 2007. p. 109-129 (Methods in Molecular Biology; Vol. 363).

Research output: Chapter in Book/Report/Conference proceedingChapter

Borgstahl, GEO 2007, How to use dynamic light scattering to improve the likelihood of growing macromolecular crystals. in S Doublie (ed.), Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallizationof Macromolecules. Methods in Molecular Biology, vol. 363, pp. 109-129. https://doi.org/10.1385/1-59745-209-2:109
Borgstahl GEO. How to use dynamic light scattering to improve the likelihood of growing macromolecular crystals. In Doublie S, editor, Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallizationof Macromolecules. 2007. p. 109-129. (Methods in Molecular Biology). https://doi.org/10.1385/1-59745-209-2:109
Borgstahl, Gloria E.O. / How to use dynamic light scattering to improve the likelihood of growing macromolecular crystals. Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallizationof Macromolecules. editor / Sylvie Doublie. 2007. pp. 109-129 (Methods in Molecular Biology).
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