Homology model for oncostatin M based on NMR structural data

Douglas Kitchen, Ross C. Huffman, Franklin J. Moy, Robert Powers

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Oncostatin M (OM) is a member of the cytokine family which regulates the proliferation and differentiation of a variety of cell types and includes interleukin-6 (IL-6), leukemia inhibitory factor (LIF), and granulocyte- colony stimulating factor (G-CSF). This family of proteins adopts a four- helix bundle fold with up-up-down-down topology and contains intramolecular disulfide bonds. Since an X-ray or NMR structure for OM is not currently available, a homology model for OM was determined from the X-ray structures of human growth hormone (hGH), LIF, and G-CSF where the alignment was based on secondary structure instead of sequence. The OM secondary structure was determined from NMR structural data, and the secondary structures for hGH, LIF, and G-CSF were obtained from the reported X-ray structures. The resulting homology model was refined using sequential NOE distance 13C restraints, chemical shift information, and a conformational database.

Original languageEnglish (US)
Pages (from-to)10581-10588
Number of pages8
JournalBiochemistry
Volume37
Issue number30
DOIs
StatePublished - Jul 28 1998

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Oncostatin M
Leukemia Inhibitory Factor
Granulocyte Colony-Stimulating Factor
Nuclear magnetic resonance
Human Growth Hormone
X-Rays
X rays
Chemical shift
Disulfides
Interleukin-6
Topology
Databases
Cytokines
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Homology model for oncostatin M based on NMR structural data. / Kitchen, Douglas; Huffman, Ross C.; Moy, Franklin J.; Powers, Robert.

In: Biochemistry, Vol. 37, No. 30, 28.07.1998, p. 10581-10588.

Research output: Contribution to journalArticle

Kitchen, D, Huffman, RC, Moy, FJ & Powers, R 1998, 'Homology model for oncostatin M based on NMR structural data', Biochemistry, vol. 37, no. 30, pp. 10581-10588. https://doi.org/10.1021/bi9727444
Kitchen, Douglas ; Huffman, Ross C. ; Moy, Franklin J. ; Powers, Robert. / Homology model for oncostatin M based on NMR structural data. In: Biochemistry. 1998 ; Vol. 37, No. 30. pp. 10581-10588.
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