Homocysteine levels during fasting and after methionine loading in adolescents with diabetic retinopathy and nephropathy

Francesco Chiarelli, Mariapina Pomilio, Angelika Mohn, Stefano Tumini, Maurizio Vanelli, Guido Morgese, Anna Spagnoli, Alberto Verrotti

Research output: Contribution to journalArticle

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Abstract

Objective: To assess plasma homocysteine levels in adolescents and young adults with type 1 (insulin-dependent) diabetes with and without microvascular complications. Study design: Homocysteine levels were measured during fasting and after methionine loading in plasma of 61 patients with onset of diabetes before the age of 12 years and duration of disease longer than 7 years. They had an albumin excretion rate (AER) between 20 and 200 μg/min in 2 of 3 overnight urine collections in a period of 6 months and/or retinopathy. Patients with persistent microalbuminuria were divided into 2 groups: subjects with AER of 20 to 70 μg/min and patients with AER of 70 to 200 μg/min. Adolescents (n = 54) without signs of diabetic retinopathy or nephropathy and matched control subjects (n = 63) were also studied. Results: Homocysteine concentrations before and after methionine load were higher in adolescents with diabetic complications than in healthy subjects (fasting values: 12.4 ± 7.9 μmol/L vs 7.8 ± 4.2 μmol/L; P < .01; after methionine load: 28.1 ± 13.2 μmol/L vs 16.6 ± 7.3 μmol/L; P < .005). Values of 11.9 μmol/L or higher were considered to constitute fasting hyperhomo-cysteinemia. The increase of homocysteine concentrations was particularly evident in young diabetic patients with AER >70 μg/min (fasting values: 14.7 ± 5.6 μmol/L; after methionine load: 34.2 ± 12.6 μmol/L) and in patients with proliferative retinopathy (fasting values: 15.1 ± 5.0 μmol/L; after methionine load: 36.8 ± 12.5 μmol/L). Conclusions: Increased plasma homocysteine concentrations may contribute to increased morbidity and death from cardiovascular disease in adolescents and young adults with diabetic retinopathy and nephropathy.

Original languageEnglish (US)
Pages (from-to)386-392
Number of pages7
JournalJournal of Pediatrics
Volume137
Issue number3
DOIs
StatePublished - Jan 1 2000

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Diabetic Nephropathies
Homocysteine
Diabetic Retinopathy
Methionine
Fasting
Albumins
Young Adult
Urine Specimen Collection
Diabetes Complications
Healthy Volunteers
Cardiovascular Diseases
Insulin
Morbidity

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Chiarelli, F., Pomilio, M., Mohn, A., Tumini, S., Vanelli, M., Morgese, G., ... Verrotti, A. (2000). Homocysteine levels during fasting and after methionine loading in adolescents with diabetic retinopathy and nephropathy. Journal of Pediatrics, 137(3), 386-392. https://doi.org/10.1067/mpd.2000.108103

Homocysteine levels during fasting and after methionine loading in adolescents with diabetic retinopathy and nephropathy. / Chiarelli, Francesco; Pomilio, Mariapina; Mohn, Angelika; Tumini, Stefano; Vanelli, Maurizio; Morgese, Guido; Spagnoli, Anna; Verrotti, Alberto.

In: Journal of Pediatrics, Vol. 137, No. 3, 01.01.2000, p. 386-392.

Research output: Contribution to journalArticle

Chiarelli, Francesco ; Pomilio, Mariapina ; Mohn, Angelika ; Tumini, Stefano ; Vanelli, Maurizio ; Morgese, Guido ; Spagnoli, Anna ; Verrotti, Alberto. / Homocysteine levels during fasting and after methionine loading in adolescents with diabetic retinopathy and nephropathy. In: Journal of Pediatrics. 2000 ; Vol. 137, No. 3. pp. 386-392.
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abstract = "Objective: To assess plasma homocysteine levels in adolescents and young adults with type 1 (insulin-dependent) diabetes with and without microvascular complications. Study design: Homocysteine levels were measured during fasting and after methionine loading in plasma of 61 patients with onset of diabetes before the age of 12 years and duration of disease longer than 7 years. They had an albumin excretion rate (AER) between 20 and 200 μg/min in 2 of 3 overnight urine collections in a period of 6 months and/or retinopathy. Patients with persistent microalbuminuria were divided into 2 groups: subjects with AER of 20 to 70 μg/min and patients with AER of 70 to 200 μg/min. Adolescents (n = 54) without signs of diabetic retinopathy or nephropathy and matched control subjects (n = 63) were also studied. Results: Homocysteine concentrations before and after methionine load were higher in adolescents with diabetic complications than in healthy subjects (fasting values: 12.4 ± 7.9 μmol/L vs 7.8 ± 4.2 μmol/L; P < .01; after methionine load: 28.1 ± 13.2 μmol/L vs 16.6 ± 7.3 μmol/L; P < .005). Values of 11.9 μmol/L or higher were considered to constitute fasting hyperhomo-cysteinemia. The increase of homocysteine concentrations was particularly evident in young diabetic patients with AER >70 μg/min (fasting values: 14.7 ± 5.6 μmol/L; after methionine load: 34.2 ± 12.6 μmol/L) and in patients with proliferative retinopathy (fasting values: 15.1 ± 5.0 μmol/L; after methionine load: 36.8 ± 12.5 μmol/L). Conclusions: Increased plasma homocysteine concentrations may contribute to increased morbidity and death from cardiovascular disease in adolescents and young adults with diabetic retinopathy and nephropathy.",
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T1 - Homocysteine levels during fasting and after methionine loading in adolescents with diabetic retinopathy and nephropathy

AU - Chiarelli, Francesco

AU - Pomilio, Mariapina

AU - Mohn, Angelika

AU - Tumini, Stefano

AU - Vanelli, Maurizio

AU - Morgese, Guido

AU - Spagnoli, Anna

AU - Verrotti, Alberto

PY - 2000/1/1

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N2 - Objective: To assess plasma homocysteine levels in adolescents and young adults with type 1 (insulin-dependent) diabetes with and without microvascular complications. Study design: Homocysteine levels were measured during fasting and after methionine loading in plasma of 61 patients with onset of diabetes before the age of 12 years and duration of disease longer than 7 years. They had an albumin excretion rate (AER) between 20 and 200 μg/min in 2 of 3 overnight urine collections in a period of 6 months and/or retinopathy. Patients with persistent microalbuminuria were divided into 2 groups: subjects with AER of 20 to 70 μg/min and patients with AER of 70 to 200 μg/min. Adolescents (n = 54) without signs of diabetic retinopathy or nephropathy and matched control subjects (n = 63) were also studied. Results: Homocysteine concentrations before and after methionine load were higher in adolescents with diabetic complications than in healthy subjects (fasting values: 12.4 ± 7.9 μmol/L vs 7.8 ± 4.2 μmol/L; P < .01; after methionine load: 28.1 ± 13.2 μmol/L vs 16.6 ± 7.3 μmol/L; P < .005). Values of 11.9 μmol/L or higher were considered to constitute fasting hyperhomo-cysteinemia. The increase of homocysteine concentrations was particularly evident in young diabetic patients with AER >70 μg/min (fasting values: 14.7 ± 5.6 μmol/L; after methionine load: 34.2 ± 12.6 μmol/L) and in patients with proliferative retinopathy (fasting values: 15.1 ± 5.0 μmol/L; after methionine load: 36.8 ± 12.5 μmol/L). Conclusions: Increased plasma homocysteine concentrations may contribute to increased morbidity and death from cardiovascular disease in adolescents and young adults with diabetic retinopathy and nephropathy.

AB - Objective: To assess plasma homocysteine levels in adolescents and young adults with type 1 (insulin-dependent) diabetes with and without microvascular complications. Study design: Homocysteine levels were measured during fasting and after methionine loading in plasma of 61 patients with onset of diabetes before the age of 12 years and duration of disease longer than 7 years. They had an albumin excretion rate (AER) between 20 and 200 μg/min in 2 of 3 overnight urine collections in a period of 6 months and/or retinopathy. Patients with persistent microalbuminuria were divided into 2 groups: subjects with AER of 20 to 70 μg/min and patients with AER of 70 to 200 μg/min. Adolescents (n = 54) without signs of diabetic retinopathy or nephropathy and matched control subjects (n = 63) were also studied. Results: Homocysteine concentrations before and after methionine load were higher in adolescents with diabetic complications than in healthy subjects (fasting values: 12.4 ± 7.9 μmol/L vs 7.8 ± 4.2 μmol/L; P < .01; after methionine load: 28.1 ± 13.2 μmol/L vs 16.6 ± 7.3 μmol/L; P < .005). Values of 11.9 μmol/L or higher were considered to constitute fasting hyperhomo-cysteinemia. The increase of homocysteine concentrations was particularly evident in young diabetic patients with AER >70 μg/min (fasting values: 14.7 ± 5.6 μmol/L; after methionine load: 34.2 ± 12.6 μmol/L) and in patients with proliferative retinopathy (fasting values: 15.1 ± 5.0 μmol/L; after methionine load: 36.8 ± 12.5 μmol/L). Conclusions: Increased plasma homocysteine concentrations may contribute to increased morbidity and death from cardiovascular disease in adolescents and young adults with diabetic retinopathy and nephropathy.

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