Homocysteine inhibits extra-embryonic vascular development in the avian embryo

Kimberly S. Latacha, Thomas H. Rosenquist

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A strong association exists between pregnancy loss and maternal elevations of the sulfur-containing amino acid, homocysteine. Because extra-embryonic vascular growth is critical to maintaining a normal pregnancy, we examined the effects of homocysteine on vessel development by exposing avian embryos to exogenous homocysteine during critical periods of vascular growth. These experiments demonstrated that homocysteine significantly reduced survival and decreased angiogenesis in the extra-embryonic vasculature. Homocysteine was also found to reduce mRNA and protein expression of vascular endothelial growth factor (VEGF), a key molecule for vascular development. Moreover, in cultured human umbilical vein endothelial cells, homocysteine increased the synthesis of nitric oxide, an important regulatory molecule for VEGF. Inhibiting the homocysteine-induced up-regulation of nitric oxide restored normal VEGF expression and vascular development. These results suggest that homocysteine may impair the development of the extra-embryonic vasculature by reducing the expression of VEGF.

Original languageEnglish (US)
Pages (from-to)323-331
Number of pages9
JournalDevelopmental Dynamics
Volume234
Issue number2
DOIs
StatePublished - Oct 1 2005

Fingerprint

Homocysteine
Embryonic Development
Blood Vessels
Embryonic Structures
Vascular Endothelial Growth Factor A
Nitric Oxide
Sulfur Amino Acids
Pregnancy
Human Umbilical Vein Endothelial Cells
Growth
Up-Regulation
Mothers
Messenger RNA

Keywords

  • Angiogenesis
  • Homocysteine
  • Nitric Oxide
  • VEGF

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Homocysteine inhibits extra-embryonic vascular development in the avian embryo. / Latacha, Kimberly S.; Rosenquist, Thomas H.

In: Developmental Dynamics, Vol. 234, No. 2, 01.10.2005, p. 323-331.

Research output: Contribution to journalArticle

@article{4d7ac656a17a488ba2b01860aee2d8ac,
title = "Homocysteine inhibits extra-embryonic vascular development in the avian embryo",
abstract = "A strong association exists between pregnancy loss and maternal elevations of the sulfur-containing amino acid, homocysteine. Because extra-embryonic vascular growth is critical to maintaining a normal pregnancy, we examined the effects of homocysteine on vessel development by exposing avian embryos to exogenous homocysteine during critical periods of vascular growth. These experiments demonstrated that homocysteine significantly reduced survival and decreased angiogenesis in the extra-embryonic vasculature. Homocysteine was also found to reduce mRNA and protein expression of vascular endothelial growth factor (VEGF), a key molecule for vascular development. Moreover, in cultured human umbilical vein endothelial cells, homocysteine increased the synthesis of nitric oxide, an important regulatory molecule for VEGF. Inhibiting the homocysteine-induced up-regulation of nitric oxide restored normal VEGF expression and vascular development. These results suggest that homocysteine may impair the development of the extra-embryonic vasculature by reducing the expression of VEGF.",
keywords = "Angiogenesis, Homocysteine, Nitric Oxide, VEGF",
author = "Latacha, {Kimberly S.} and Rosenquist, {Thomas H.}",
year = "2005",
month = "10",
day = "1",
doi = "10.1002/dvdy.20527",
language = "English (US)",
volume = "234",
pages = "323--331",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Homocysteine inhibits extra-embryonic vascular development in the avian embryo

AU - Latacha, Kimberly S.

AU - Rosenquist, Thomas H.

PY - 2005/10/1

Y1 - 2005/10/1

N2 - A strong association exists between pregnancy loss and maternal elevations of the sulfur-containing amino acid, homocysteine. Because extra-embryonic vascular growth is critical to maintaining a normal pregnancy, we examined the effects of homocysteine on vessel development by exposing avian embryos to exogenous homocysteine during critical periods of vascular growth. These experiments demonstrated that homocysteine significantly reduced survival and decreased angiogenesis in the extra-embryonic vasculature. Homocysteine was also found to reduce mRNA and protein expression of vascular endothelial growth factor (VEGF), a key molecule for vascular development. Moreover, in cultured human umbilical vein endothelial cells, homocysteine increased the synthesis of nitric oxide, an important regulatory molecule for VEGF. Inhibiting the homocysteine-induced up-regulation of nitric oxide restored normal VEGF expression and vascular development. These results suggest that homocysteine may impair the development of the extra-embryonic vasculature by reducing the expression of VEGF.

AB - A strong association exists between pregnancy loss and maternal elevations of the sulfur-containing amino acid, homocysteine. Because extra-embryonic vascular growth is critical to maintaining a normal pregnancy, we examined the effects of homocysteine on vessel development by exposing avian embryos to exogenous homocysteine during critical periods of vascular growth. These experiments demonstrated that homocysteine significantly reduced survival and decreased angiogenesis in the extra-embryonic vasculature. Homocysteine was also found to reduce mRNA and protein expression of vascular endothelial growth factor (VEGF), a key molecule for vascular development. Moreover, in cultured human umbilical vein endothelial cells, homocysteine increased the synthesis of nitric oxide, an important regulatory molecule for VEGF. Inhibiting the homocysteine-induced up-regulation of nitric oxide restored normal VEGF expression and vascular development. These results suggest that homocysteine may impair the development of the extra-embryonic vasculature by reducing the expression of VEGF.

KW - Angiogenesis

KW - Homocysteine

KW - Nitric Oxide

KW - VEGF

UR - http://www.scopus.com/inward/record.url?scp=25444486633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25444486633&partnerID=8YFLogxK

U2 - 10.1002/dvdy.20527

DO - 10.1002/dvdy.20527

M3 - Article

C2 - 16124006

AN - SCOPUS:25444486633

VL - 234

SP - 323

EP - 331

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 2

ER -