Abstract

Hog barn workers have an increased incidence of respiratory tract symptoms and demonstrate an increase in lung inflammatory mediators, including interleukin (IL)-8 and IL-6. Utilizing direct kinase assays for protein kinase C (PKC) activation, we demonstrated that dust from hog confinement facilities, or hog dust extract (HDE), augments PKC activity of human airway epithelial cells in vitro. A 5% dilution of HDE typically stimulates an approximately twofold increase in human bronchial epithelial cell (HBEC) PKC activity compared with control medium-treated cells. This increase in PKC is observed with 15 min of HDE treatment, and kinase activity reaches peak activity by 1-2 h of HDE treatment before returning to baseline PKC levels between 6 and 24 h. The classic PKC inhibitor, calphostin C, blocks HDE-stimulated PKC activity and associated IL-8 and IL-6 release. Desensitization to HDE stimulation of PKC activation does not appear to occur because subsequent exposures to HDE after an initial exposure result in further augmentation of PKC. Detoxification of HDE with polymyxin B to remove endotoxin did not change PKC activation or IL-8 release, suggesting that endotoxin is not solely responsible for HDE augmentation of PKC. These data support the hypothesis that HDE exposure augments HBEC IL-8 and IL-6 release via a PKC-dependent pathway.

Original languageEnglish (US)
Pages (from-to)289-296
Number of pages8
JournalJournal of Applied Physiology
Volume93
Issue number1
DOIs
StatePublished - Jan 1 2002

Fingerprint

Dust
Interleukin-8
Protein Kinase C
Interleukin-6
Epithelial Cells
Endotoxins
Polymyxin B
Protein C Inhibitor
Protein Kinase Inhibitors
Human Activities
Respiratory System
Phosphotransferases
Lung
Incidence

Keywords

  • Airway
  • Cytokines
  • Endotoxin
  • Interleukin
  • Kinase activity
  • Lipopolysaccharide
  • Lung

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Hog barn dust extract stimulates IL-8 and IL-6 release in human bronchial epithelial cells via PKC activation. / Romberger, Debra; Bodlak, V.; Von Essen, Susanna G; Mathisen, T.; Wyatt, Todd A.

In: Journal of Applied Physiology, Vol. 93, No. 1, 01.01.2002, p. 289-296.

Research output: Contribution to journalArticle

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abstract = "Hog barn workers have an increased incidence of respiratory tract symptoms and demonstrate an increase in lung inflammatory mediators, including interleukin (IL)-8 and IL-6. Utilizing direct kinase assays for protein kinase C (PKC) activation, we demonstrated that dust from hog confinement facilities, or hog dust extract (HDE), augments PKC activity of human airway epithelial cells in vitro. A 5{\%} dilution of HDE typically stimulates an approximately twofold increase in human bronchial epithelial cell (HBEC) PKC activity compared with control medium-treated cells. This increase in PKC is observed with 15 min of HDE treatment, and kinase activity reaches peak activity by 1-2 h of HDE treatment before returning to baseline PKC levels between 6 and 24 h. The classic PKC inhibitor, calphostin C, blocks HDE-stimulated PKC activity and associated IL-8 and IL-6 release. Desensitization to HDE stimulation of PKC activation does not appear to occur because subsequent exposures to HDE after an initial exposure result in further augmentation of PKC. Detoxification of HDE with polymyxin B to remove endotoxin did not change PKC activation or IL-8 release, suggesting that endotoxin is not solely responsible for HDE augmentation of PKC. These data support the hypothesis that HDE exposure augments HBEC IL-8 and IL-6 release via a PKC-dependent pathway.",
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AU - Wyatt, Todd A

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