HIV promoter activity in primary antigen-specific human T lymphocytes

R. T. Horvat, Charles Wood

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Human retroviruses, such as the HIV, infects human T cells, and efficient HIV replication occurs primarily in activated T cells rather than resting cells. Increased HIV production is likely caused by the activation of the retroviral promoter, and the HIV promoter may be regulated by intracellular signals induced during immune stimulation. To examine the regulation of retroviral promoter activity in normal, Ag-specific primary T lymphoblasts, a heterogeneous population of primary human T cells was transfected with either the HIV promoter or a promoter from a different retrovirus, Rous sarcoma virus (RSV) by protoplast fusion technique. Transfected T cells responded normally to Ag or mitogen stimulation, and activation of these T cells increased both the HIV and RSV promoter activity. Promoter activity was assessed by using transient expression assays after the T cells were restimulated with Ag, mitogen, or IL-2. In situ hybridization of transfected human T cells showed that 68 to 95% of activated lymphocytes expressed CAT mRNA directed by HIV or RSV. Thus, protoplast transfection of primary T cells was efficient in that the majority of cells expressed CAT message. By deletion of different regions of the HIV promoter, the enhancer region was identified as necessary for effective HIV promoter activity. In addition these deletion studies indentified a region that negatively affects HIV promoter activity in primary T cells. Cotransfection of the HIV promoter with the HIV transactivator protein, tat, increases HIV promoter activity in both resting and activated primary human T cells only when the tat target sequences were present.

Original languageEnglish (US)
Pages (from-to)2745-2751
Number of pages7
JournalJournal of Immunology
Volume143
Issue number8
StatePublished - Jan 1 1989

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HIV
T-Lymphocytes
Antigens
Rous sarcoma virus
Protoplasts
Retroviridae
Mitogens
HIV Enhancer
Human Immunodeficiency Virus rev Gene Products
Genetic Promoter Regions
Interleukin-2
In Situ Hybridization
Transfection
Lymphocytes
Messenger RNA
Population

ASJC Scopus subject areas

  • Immunology

Cite this

HIV promoter activity in primary antigen-specific human T lymphocytes. / Horvat, R. T.; Wood, Charles.

In: Journal of Immunology, Vol. 143, No. 8, 01.01.1989, p. 2745-2751.

Research output: Contribution to journalArticle

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