HIV infection of choroid plexus in AIDS and asymptomatic HIV-infected patients suggests that the choroid plexus may be a reservoir of productive infection

Carol K. Petito, Hexin Chen, Angeline R. Mastri, Jorge Torres-Munoz, Brenda Roberts, Charles Wood

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The choroid plexus (CPx) may be an important site of viral dissemination since monocytes and dendritic cells in its stroma are infected with HIV in AIDS patients and since the ratio of CPx to brain infection is more than 2:1. In order to see if CPx infection also develops in asymptomatic (ASY) HIV-infected patients, we examined archival formalin-fixed brain and CPx from 14 AIDS and seven ASY cases, using routine histology, immunohistochemistry for HIV gp41, and DNA extraction and gene amplification for HIV DNA. Eight of 14 AIDS (57%) had HIV-positive cells in the CPx and four (29%) had HIV encephalitis. Two of seven ASY cases (29%) had HIV-positive cells in the CPx but none had HIV encephalitis. Extracted DNA from brain, CPx and systemic organs of five ASY cases was amplified by nested PCR with or without Southern blotting for HIV env gene. It was positive in systemic organs in five cases; in CPx in four cases; and in brain in one case. This study shows that the CPx is a site of HIV infection in ASY patients and that the frequency of CPx infection is higher than seen in brain in both AIDS and ASY cases. The results are consistent with the hypothesis that the CPx may be a site for hematogeneous spread and a reservoir for HIV infection during the period of clinical latency.

Original languageEnglish (US)
Pages (from-to)670-677
Number of pages8
JournalJournal of NeuroVirology
Volume5
Issue number6
DOIs
StatePublished - Jan 1 1999

Fingerprint

Choroid Plexus
HIV Infections
Acquired Immunodeficiency Syndrome
HIV
Infection
Brain
Encephalitis
DNA
HIV Envelope Protein gp41
env Genes
Gene Amplification
Southern Blotting
Dendritic Cells
Formaldehyde
Monocytes
Histology
Immunohistochemistry

Keywords

  • Brain
  • Immunohistochemistry
  • PCR
  • Viral reservoir

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Virology

Cite this

HIV infection of choroid plexus in AIDS and asymptomatic HIV-infected patients suggests that the choroid plexus may be a reservoir of productive infection. / Petito, Carol K.; Chen, Hexin; Mastri, Angeline R.; Torres-Munoz, Jorge; Roberts, Brenda; Wood, Charles.

In: Journal of NeuroVirology, Vol. 5, No. 6, 01.01.1999, p. 670-677.

Research output: Contribution to journalArticle

Petito, Carol K. ; Chen, Hexin ; Mastri, Angeline R. ; Torres-Munoz, Jorge ; Roberts, Brenda ; Wood, Charles. / HIV infection of choroid plexus in AIDS and asymptomatic HIV-infected patients suggests that the choroid plexus may be a reservoir of productive infection. In: Journal of NeuroVirology. 1999 ; Vol. 5, No. 6. pp. 670-677.
@article{c998a3f2eb5041e6b6a8c534d2532126,
title = "HIV infection of choroid plexus in AIDS and asymptomatic HIV-infected patients suggests that the choroid plexus may be a reservoir of productive infection",
abstract = "The choroid plexus (CPx) may be an important site of viral dissemination since monocytes and dendritic cells in its stroma are infected with HIV in AIDS patients and since the ratio of CPx to brain infection is more than 2:1. In order to see if CPx infection also develops in asymptomatic (ASY) HIV-infected patients, we examined archival formalin-fixed brain and CPx from 14 AIDS and seven ASY cases, using routine histology, immunohistochemistry for HIV gp41, and DNA extraction and gene amplification for HIV DNA. Eight of 14 AIDS (57{\%}) had HIV-positive cells in the CPx and four (29{\%}) had HIV encephalitis. Two of seven ASY cases (29{\%}) had HIV-positive cells in the CPx but none had HIV encephalitis. Extracted DNA from brain, CPx and systemic organs of five ASY cases was amplified by nested PCR with or without Southern blotting for HIV env gene. It was positive in systemic organs in five cases; in CPx in four cases; and in brain in one case. This study shows that the CPx is a site of HIV infection in ASY patients and that the frequency of CPx infection is higher than seen in brain in both AIDS and ASY cases. The results are consistent with the hypothesis that the CPx may be a site for hematogeneous spread and a reservoir for HIV infection during the period of clinical latency.",
keywords = "Brain, Immunohistochemistry, PCR, Viral reservoir",
author = "Petito, {Carol K.} and Hexin Chen and Mastri, {Angeline R.} and Jorge Torres-Munoz and Brenda Roberts and Charles Wood",
year = "1999",
month = "1",
day = "1",
doi = "10.3109/13550289909021295",
language = "English (US)",
volume = "5",
pages = "670--677",
journal = "Journal of NeuroVirology",
issn = "1355-0284",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - HIV infection of choroid plexus in AIDS and asymptomatic HIV-infected patients suggests that the choroid plexus may be a reservoir of productive infection

AU - Petito, Carol K.

AU - Chen, Hexin

AU - Mastri, Angeline R.

AU - Torres-Munoz, Jorge

AU - Roberts, Brenda

AU - Wood, Charles

PY - 1999/1/1

Y1 - 1999/1/1

N2 - The choroid plexus (CPx) may be an important site of viral dissemination since monocytes and dendritic cells in its stroma are infected with HIV in AIDS patients and since the ratio of CPx to brain infection is more than 2:1. In order to see if CPx infection also develops in asymptomatic (ASY) HIV-infected patients, we examined archival formalin-fixed brain and CPx from 14 AIDS and seven ASY cases, using routine histology, immunohistochemistry for HIV gp41, and DNA extraction and gene amplification for HIV DNA. Eight of 14 AIDS (57%) had HIV-positive cells in the CPx and four (29%) had HIV encephalitis. Two of seven ASY cases (29%) had HIV-positive cells in the CPx but none had HIV encephalitis. Extracted DNA from brain, CPx and systemic organs of five ASY cases was amplified by nested PCR with or without Southern blotting for HIV env gene. It was positive in systemic organs in five cases; in CPx in four cases; and in brain in one case. This study shows that the CPx is a site of HIV infection in ASY patients and that the frequency of CPx infection is higher than seen in brain in both AIDS and ASY cases. The results are consistent with the hypothesis that the CPx may be a site for hematogeneous spread and a reservoir for HIV infection during the period of clinical latency.

AB - The choroid plexus (CPx) may be an important site of viral dissemination since monocytes and dendritic cells in its stroma are infected with HIV in AIDS patients and since the ratio of CPx to brain infection is more than 2:1. In order to see if CPx infection also develops in asymptomatic (ASY) HIV-infected patients, we examined archival formalin-fixed brain and CPx from 14 AIDS and seven ASY cases, using routine histology, immunohistochemistry for HIV gp41, and DNA extraction and gene amplification for HIV DNA. Eight of 14 AIDS (57%) had HIV-positive cells in the CPx and four (29%) had HIV encephalitis. Two of seven ASY cases (29%) had HIV-positive cells in the CPx but none had HIV encephalitis. Extracted DNA from brain, CPx and systemic organs of five ASY cases was amplified by nested PCR with or without Southern blotting for HIV env gene. It was positive in systemic organs in five cases; in CPx in four cases; and in brain in one case. This study shows that the CPx is a site of HIV infection in ASY patients and that the frequency of CPx infection is higher than seen in brain in both AIDS and ASY cases. The results are consistent with the hypothesis that the CPx may be a site for hematogeneous spread and a reservoir for HIV infection during the period of clinical latency.

KW - Brain

KW - Immunohistochemistry

KW - PCR

KW - Viral reservoir

UR - http://www.scopus.com/inward/record.url?scp=0033378330&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033378330&partnerID=8YFLogxK

U2 - 10.3109/13550289909021295

DO - 10.3109/13550289909021295

M3 - Article

VL - 5

SP - 670

EP - 677

JO - Journal of NeuroVirology

JF - Journal of NeuroVirology

SN - 1355-0284

IS - 6

ER -