HIV-1 transforms the monocyte plasma membrane proteome

Irena Kadiu, Tong Wang, Joshua D. Schlautman, Larisa Dubrovsky, Pawel S Ciborowski, Michael Bukrinsky, Howard Eliot Gendelman

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

How HIV-1 affects the monocyte proteome is incompletely understood. We posit that one functional consequence of virus-exposure to the monocyte is the facilitation of protein transformation from the cytosol to the plasma membrane (PM). To test this, cell surface labeling with CyDye fluorophores followed by 2 dimensional differential in-gel electrophoresis (2D DIGE) and liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed. Fifty three percent of HIV-1 induced proteins were PM associated. These were linked, in large measure, to cellular activation and oxidative stress. They included, but not limited to, biliverdin reductase, leukotriene hydrolase A4, heat shock protein 70, and cystatin B. HIV-1 induced PM protein translocation was associated with cathepsin B- and caspase 9, 3-dependent apoptosis. In contrast, PMA-treated monocytes bypassed caspase 3, 9 pathways and lead to cathepsin B-dependent necrosis. These results demonstrate that HIV-1 uniquely affects monocyte activation and oxidative stress. These do not affect viral infection dynamics but are linked to stress-induced cell death.

Original languageEnglish (US)
Pages (from-to)44-58
Number of pages15
JournalCellular Immunology
Volume258
Issue number1
DOIs
StatePublished - Apr 8 2009

Fingerprint

Proteome
HIV-1
Monocytes
Cell Membrane
Cathepsin B
Caspase 9
biliverdin reductase
Caspase 3
Cystatin B
Oxidative Stress
HSP70 Heat-Shock Proteins
Protein Transport
Virus Diseases
Tandem Mass Spectrometry
Liquid Chromatography
Cytosol
Gel Chromatography
Electrophoresis
Blood Proteins
Membrane Proteins

Keywords

  • Apoptosis
  • HIV-1
  • Monocytes
  • Necrosis
  • Oxidative stress
  • Phorbol myristate acetate
  • Plasma membrane
  • Proteomics
  • Vitamin E

ASJC Scopus subject areas

  • Immunology

Cite this

HIV-1 transforms the monocyte plasma membrane proteome. / Kadiu, Irena; Wang, Tong; Schlautman, Joshua D.; Dubrovsky, Larisa; Ciborowski, Pawel S; Bukrinsky, Michael; Gendelman, Howard Eliot.

In: Cellular Immunology, Vol. 258, No. 1, 08.04.2009, p. 44-58.

Research output: Contribution to journalArticle

Kadiu, I, Wang, T, Schlautman, JD, Dubrovsky, L, Ciborowski, PS, Bukrinsky, M & Gendelman, HE 2009, 'HIV-1 transforms the monocyte plasma membrane proteome', Cellular Immunology, vol. 258, no. 1, pp. 44-58. https://doi.org/10.1016/j.cellimm.2009.03.012
Kadiu I, Wang T, Schlautman JD, Dubrovsky L, Ciborowski PS, Bukrinsky M et al. HIV-1 transforms the monocyte plasma membrane proteome. Cellular Immunology. 2009 Apr 8;258(1):44-58. https://doi.org/10.1016/j.cellimm.2009.03.012
Kadiu, Irena ; Wang, Tong ; Schlautman, Joshua D. ; Dubrovsky, Larisa ; Ciborowski, Pawel S ; Bukrinsky, Michael ; Gendelman, Howard Eliot. / HIV-1 transforms the monocyte plasma membrane proteome. In: Cellular Immunology. 2009 ; Vol. 258, No. 1. pp. 44-58.
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