HIV-1 Tat co-operates with IFN-γ and TNF-α to increase CXCL10 in human astrocytes

Rachel Williams, Honghong Yao, Navneet K. Dhillon, Shilpa J. Buch

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

HIV-associated neurological disorders (HAND) are estimated to affect 60% of the HIV infected population. HIV-encephalitis (HIVE), the pathological correlate of the most severe form of HAND is often characterized by glial activation, cytokine/chemokine dysregulation, and neuronal damage and loss. However, the severity of HIVE correlates better with glial activation rather than viral load. One of the characteristic features of HIVE is the increased amount of the neurotoxic chemokine, CXCL10. This chemokine can be released from astroglia activated with the pro-inflammatory cytokines IFN-γ and TNF-α, in conjunction with HIV-1 Tat, all of which are elevated in HIVE. In an effort to understand the pathogenesis of HAND, this study was aimed at exploring the regulation of CXCL10 by cellular and viral factors during astrocyte activation. Specifically, the data herein demonstrate that the combined actions of HIV-1 Tat and the pro-inflammatory cytokines, IFN-γ and TNF-α, result in the induction of CXCL10 at both the RNA and protein level. Furthermore, CXCL10 induction was found to be regulated transcriptionally by the activation of the p38, Jnk, and Akt signaling pathways and their downstream transcription factors, NF-κB and STAT-1α. Since CXCL10 levels are linked to disease severity, understanding its regulation could aid in the development of therapeutic intervention strategies for HAND.

Original languageEnglish (US)
Article numbere5709
JournalPloS one
Volume4
Issue number5
DOIs
StatePublished - May 28 2009

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nervous system diseases
astrocytes
encephalitis
Human immunodeficiency virus 1
Astrocytes
HIV-1
Chemical activation
HIV
cytokines
neuroglia
chemokines
Cytokines
Chemokines
Encephalitis
Nervous System Diseases
Chemokine CXCL10
development aid
viral load
disease severity
Neuroglia

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

HIV-1 Tat co-operates with IFN-γ and TNF-α to increase CXCL10 in human astrocytes. / Williams, Rachel; Yao, Honghong; Dhillon, Navneet K.; Buch, Shilpa J.

In: PloS one, Vol. 4, No. 5, e5709, 28.05.2009.

Research output: Contribution to journalArticle

Williams, Rachel ; Yao, Honghong ; Dhillon, Navneet K. ; Buch, Shilpa J. / HIV-1 Tat co-operates with IFN-γ and TNF-α to increase CXCL10 in human astrocytes. In: PloS one. 2009 ; Vol. 4, No. 5.
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