HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome: New insights into blood-brain barrier dysfunction for HIV-1-associated dementia

Mary Ricardo-Dukelow, Irena Kadiu, Wojciech Rozek, Joshua Schlautman, Yuri Persidsky, Pawel S Ciborowski, Georgette D Kanmogne, Howard Eliot Gendelman

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Blood-brain barrier (BBB) compromise and transendothelial migration of HIV-infected leukocytes into the central nervous system (CNS) underlies the neuropathogenesis of HIV-1 infection. How this occurs is incompletely understood. We used a proteomic platform integrating difference gel electrophoresis and tandem mass spectrometry peptide sequencing to determine the effects that HIV-1-infected macrophages have on human brain microvascular endothelial cell (HBMEC) protein profiles. HIV-1 infected monocyte-derived macrophages (MDM) induced the upregulation of over 200 HBMEC proteins. These included metabolic, voltage-gated ion channels, heat shock, transport, cytoskeletal, regulatory, and calcium binding proteins. Results were validated by Western blot analysis. We conclude that HIV-1-infected MDM affect the HBMEC proteome and, in this way, affect BBB dysfunction and the development of HIV-1 CNS disease.

Original languageEnglish (US)
Pages (from-to)37-46
Number of pages10
JournalJournal of Neuroimmunology
Volume185
Issue number1-2
DOIs
StatePublished - Apr 1 2007

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Proteome
Blood-Brain Barrier
Dementia
HIV-1
Endothelial Cells
Macrophages
Brain
Transendothelial and Transepithelial Migration
Calcium-Binding Proteins
Central Nervous System Diseases
Tandem Mass Spectrometry
Ion Channels
Proteomics
HIV Infections
Electrophoresis
Shock
Proteins
Leukocytes
Up-Regulation
Central Nervous System

Keywords

  • 2D difference gel electrophoresis
  • Human brain microvascular endothelial cells
  • Human immunodeficiency virus
  • Monocyte-derived macrophages
  • Proteomics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

HIV-1 infected monocyte-derived macrophages affect the human brain microvascular endothelial cell proteome : New insights into blood-brain barrier dysfunction for HIV-1-associated dementia. / Ricardo-Dukelow, Mary; Kadiu, Irena; Rozek, Wojciech; Schlautman, Joshua; Persidsky, Yuri; Ciborowski, Pawel S; Kanmogne, Georgette D; Gendelman, Howard Eliot.

In: Journal of Neuroimmunology, Vol. 185, No. 1-2, 01.04.2007, p. 37-46.

Research output: Contribution to journalArticle

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