HIV-1-infected astrocytes and the microglial proteome

Tong Wang, Nan Gong, Jianuo Liu, Irena Kadiu, Stephanie D. Kraft-Terry, Joshua D. Schlautman, Pawel S Ciborowski, David J. Volsky, Howard Eliot Gendelman

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The human immunodeficiency virus (HIV) invades the central nervous system early after viral exposure but causes progressive cognitive, behavior, and motor impairments years later with the onset of immune deficiency. Although in the brain, HIV preferentially replicates productively in cells of mononuclear phagocyte (MP; blood borne macrophage and microglia), astrocytes also can be infected, at low and variable frequency, particularly in patients with encephalitis. Among their many functions, astrocytes network with microglia to provide the first line of defense against microbial infection; however, very little is known about astrocytes' consequences on MP. Here, we addressed this question using co-culture systems of HIV-infected mouse astrocytes and microglia. Pseudotyped vesicular stomatis virus/HIV was used to circumvent the absence of viral receptors and ensure cell genotypic uniformity for studies of intercellular communication. The study demonstrated that infected astrocytes show modest changes in protein elements compared to uninfected cells. In contrast, infected astrocytes induce robust changes in the proteome of HIV-1-infected microglia. Accelerated cell death and redox proteins, among others, were produced in abundance. The observations confirmed the potential of astrocytes to influence the neuropathogenesis of HIV-1 infection by specifically altering the neurotoxic potential of infected microglia and regulating viral maturation.

Original languageEnglish (US)
Pages (from-to)173-186
Number of pages14
JournalJournal of Neuroimmune Pharmacology
Volume3
Issue number3
DOIs
StatePublished - Sep 1 2008

Fingerprint

Proteome
Astrocytes
HIV-1
Microglia
HIV
Virus Diseases
Encephalitis
Phagocytes
Coculture Techniques
Oxidation-Reduction
Proteins
Cell Death
Central Nervous System
Macrophages
Viruses
Brain
Infection

Keywords

  • Astrocytes
  • Cell mobility
  • Human immunodeficiency virus
  • Microglia
  • Neurotoxicity
  • Proteomics
  • Pseudotyped viral infection

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

Wang, T., Gong, N., Liu, J., Kadiu, I., Kraft-Terry, S. D., Schlautman, J. D., ... Gendelman, H. E. (2008). HIV-1-infected astrocytes and the microglial proteome. Journal of Neuroimmune Pharmacology, 3(3), 173-186. https://doi.org/10.1007/s11481-008-9110-x

HIV-1-infected astrocytes and the microglial proteome. / Wang, Tong; Gong, Nan; Liu, Jianuo; Kadiu, Irena; Kraft-Terry, Stephanie D.; Schlautman, Joshua D.; Ciborowski, Pawel S; Volsky, David J.; Gendelman, Howard Eliot.

In: Journal of Neuroimmune Pharmacology, Vol. 3, No. 3, 01.09.2008, p. 173-186.

Research output: Contribution to journalArticle

Wang, T, Gong, N, Liu, J, Kadiu, I, Kraft-Terry, SD, Schlautman, JD, Ciborowski, PS, Volsky, DJ & Gendelman, HE 2008, 'HIV-1-infected astrocytes and the microglial proteome', Journal of Neuroimmune Pharmacology, vol. 3, no. 3, pp. 173-186. https://doi.org/10.1007/s11481-008-9110-x
Wang T, Gong N, Liu J, Kadiu I, Kraft-Terry SD, Schlautman JD et al. HIV-1-infected astrocytes and the microglial proteome. Journal of Neuroimmune Pharmacology. 2008 Sep 1;3(3):173-186. https://doi.org/10.1007/s11481-008-9110-x
Wang, Tong ; Gong, Nan ; Liu, Jianuo ; Kadiu, Irena ; Kraft-Terry, Stephanie D. ; Schlautman, Joshua D. ; Ciborowski, Pawel S ; Volsky, David J. ; Gendelman, Howard Eliot. / HIV-1-infected astrocytes and the microglial proteome. In: Journal of Neuroimmune Pharmacology. 2008 ; Vol. 3, No. 3. pp. 173-186.
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