Histone chaperone ASF1A is required for maintenance of pluripotency and cellular reprogramming

Elena Gonzalez-Muñoz, Yohanna Arboleda-Estudillo, Hasan H. Otu, Jose B. Cibelli

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Unfertilized oocytes have the intrinsic capacity to remodel sperm and the nuclei of somatic cells. The discoveries that cells can change their phenotype from differentiated to embryonic state using oocytes or specific transcription factors have been recognized as two major breakthroughs in the biomedical field. Here, we show that ASF1A, a histone-remodeling chaperone specifically enriched in the metaphase II human oocyte, is necessary for reprogramming of human adult dermal fibroblasts (hADFs) into undifferentiated induced pluripotent stem cell. We also show that overexpression of just ASF1A and OCT4 in hADFs exposed to the oocyte-specific paracrine growth factor GDF9 can reprogram hADFs into pluripotent cells. Our Report underscores the importance of studying the unfertilized MII oocyte as a means to understand the molecular pathways governing somatic cell reprogramming.

Original languageEnglish (US)
Pages (from-to)822-825
Number of pages4
JournalScience
Volume345
Issue number6198
DOIs
StatePublished - Aug 15 2014

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Histone Chaperones
Oocytes
Maintenance
Fibroblasts
Skin
Induced Pluripotent Stem Cells
Metaphase
Cell Nucleus
Spermatozoa
Intercellular Signaling Peptides and Proteins
Transcription Factors
Cellular Reprogramming
Phenotype

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Histone chaperone ASF1A is required for maintenance of pluripotency and cellular reprogramming. / Gonzalez-Muñoz, Elena; Arboleda-Estudillo, Yohanna; Otu, Hasan H.; Cibelli, Jose B.

In: Science, Vol. 345, No. 6198, 15.08.2014, p. 822-825.

Research output: Contribution to journalArticle

Gonzalez-Muñoz, E, Arboleda-Estudillo, Y, Otu, HH & Cibelli, JB 2014, 'Histone chaperone ASF1A is required for maintenance of pluripotency and cellular reprogramming', Science, vol. 345, no. 6198, pp. 822-825. https://doi.org/10.1126/science.1254745
Gonzalez-Muñoz, Elena ; Arboleda-Estudillo, Yohanna ; Otu, Hasan H. ; Cibelli, Jose B. / Histone chaperone ASF1A is required for maintenance of pluripotency and cellular reprogramming. In: Science. 2014 ; Vol. 345, No. 6198. pp. 822-825.
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