Histone biotinylation in Candida albicans

Sahar Hasim, Swetha Tati, Nandakumar Madayiputhiya, Renu Nandakumar, Kenneth Nickerson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Candida albicans is an opportunistic fungal pathogen in humans. It is a polymorphic fungus: it can live as yeasts, hyphae, or pseudohyphae. Biotin is required for cell growth and fatty acid metabolism because it is used as a cofactor for carboxylases such as acetyl-CoA carboxylase, and pyruvate carboxylase. In addition, we have discovered that biotin is used to modify histones in C. albicans. Biotinylation was detected by Western blots using a monoclonal antibiotin HRP-conjugated antibody as well as with qTOF and LC/MS/MS mass spectrometry. As a precaution, the antibiotin antibody was dialyzed against neutravidin prior to use. During this study, we observed that three histones, H2A, H2B, and H4, were biotinylated at many lysine residues in an apparently nonsite-specific manner. Roughly, equivalent levels of acetylation, methylation, and phosphorylation were found in histones from biotin-replete and biotin-starved cells, but histone biotinylation was only observed for cells grown in excess biotin. The function of histone biotinylation in C. albicans is still unknown but, because C. albicans is a natural biotin auxotroph, a storage reservoir for biotin is attractive. Techniques used to detect histone biotinylation in C. albicans did not detect any histone biotinylation in Saccharomyces cerevisiae.

Original languageEnglish (US)
Pages (from-to)529-539
Number of pages11
JournalFEMS Yeast Research
Volume13
Issue number6
DOIs
StatePublished - Sep 1 2013

Fingerprint

Biotinylation
Biotin
Candida albicans
Histones
Pyruvate Carboxylase
Acetyl-CoA Carboxylase
Hyphae
Antibodies
Acetylation
Methylation
Lysine
Saccharomyces cerevisiae
Mass Spectrometry
Fungi
Fatty Acids
Yeasts
Western Blotting
Phosphorylation
Growth

Keywords

  • Anti-biotin antibody
  • Arc1p
  • Biotin storage
  • Candida sphaeroplasts
  • LC/MS/MS

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Hasim, S., Tati, S., Madayiputhiya, N., Nandakumar, R., & Nickerson, K. (2013). Histone biotinylation in Candida albicans. FEMS Yeast Research, 13(6), 529-539. https://doi.org/10.1111/1567-1364.12056

Histone biotinylation in Candida albicans. / Hasim, Sahar; Tati, Swetha; Madayiputhiya, Nandakumar; Nandakumar, Renu; Nickerson, Kenneth.

In: FEMS Yeast Research, Vol. 13, No. 6, 01.09.2013, p. 529-539.

Research output: Contribution to journalArticle

Hasim, S, Tati, S, Madayiputhiya, N, Nandakumar, R & Nickerson, K 2013, 'Histone biotinylation in Candida albicans', FEMS Yeast Research, vol. 13, no. 6, pp. 529-539. https://doi.org/10.1111/1567-1364.12056
Hasim S, Tati S, Madayiputhiya N, Nandakumar R, Nickerson K. Histone biotinylation in Candida albicans. FEMS Yeast Research. 2013 Sep 1;13(6):529-539. https://doi.org/10.1111/1567-1364.12056
Hasim, Sahar ; Tati, Swetha ; Madayiputhiya, Nandakumar ; Nandakumar, Renu ; Nickerson, Kenneth. / Histone biotinylation in Candida albicans. In: FEMS Yeast Research. 2013 ; Vol. 13, No. 6. pp. 529-539.
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