Histone acetyltransferase p300 acetylates Pax5 and strongly enhances Pax5-mediated transcriptional activity

Ti He, Sang Yong Hong, Lin Huang, Weihua Xue, Zhihong Yu, Hyoung Kwon, Marion Kirk, Shi Jian Ding, Kaihong Su, Zhixin Zhang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Pax5/B cell lineage specific activator protein (BSAP) is a B lineage-specific regulator that controls the B lineage-specific gene expression program and immunoglobulin gene VH to DJH recombination. Despite extensive studies on its multiple functions, little is known about how the activity of Pax5 is regulated. Here, we show that co-expression of histone acetyltransferase E1A binding protein p300 dramatically enhances Pax5-mediated transcriptional activation. The p300-mediated enhancement is dependent on its intrinsic histone acetyltransferase activity. Moreover, p300 interacts with the C terminus of Pax5 and acetylates multiple lysine residues within the paired box DNA binding domain of Pax5. Mutations of lysine residues 67 and 87/89 to alanine within Pax5 abolish p300-mediated enhancement of Pax5-induced Luc-CD19 reporter expression in HEK293 cells and prevent Pax5 to activate endogenous Cd19 and Blnk expression in Pax5-/- murine pro B cells. These results uncover a novel level of regulation of Pax5 function by p300-mediated acetylation.

Original languageEnglish (US)
Pages (from-to)14137-14145
Number of pages9
JournalJournal of Biological Chemistry
Volume286
Issue number16
DOIs
StatePublished - Apr 22 2011

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Histone Acetyltransferases
Lysine
PAX5 Transcription Factor
Cells
Acetylation
Immunoglobulin Genes
B-Lymphoid Precursor Cells
HEK293 Cells
Cell Lineage
Gene expression
Alanine
Transcriptional Activation
Genetic Recombination
Immunoglobulins
Carrier Proteins
Genes
Chemical activation
Gene Expression
Mutation
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Histone acetyltransferase p300 acetylates Pax5 and strongly enhances Pax5-mediated transcriptional activity. / He, Ti; Hong, Sang Yong; Huang, Lin; Xue, Weihua; Yu, Zhihong; Kwon, Hyoung; Kirk, Marion; Ding, Shi Jian; Su, Kaihong; Zhang, Zhixin.

In: Journal of Biological Chemistry, Vol. 286, No. 16, 22.04.2011, p. 14137-14145.

Research output: Contribution to journalArticle

He, T, Hong, SY, Huang, L, Xue, W, Yu, Z, Kwon, H, Kirk, M, Ding, SJ, Su, K & Zhang, Z 2011, 'Histone acetyltransferase p300 acetylates Pax5 and strongly enhances Pax5-mediated transcriptional activity', Journal of Biological Chemistry, vol. 286, no. 16, pp. 14137-14145. https://doi.org/10.1074/jbc.M110.176289
He, Ti ; Hong, Sang Yong ; Huang, Lin ; Xue, Weihua ; Yu, Zhihong ; Kwon, Hyoung ; Kirk, Marion ; Ding, Shi Jian ; Su, Kaihong ; Zhang, Zhixin. / Histone acetyltransferase p300 acetylates Pax5 and strongly enhances Pax5-mediated transcriptional activity. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 16. pp. 14137-14145.
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