Histamine production by Klebsiella pneumoniae and an incident of scombroid fish poisoning

S. L. Taylor, L. S. Guthertz, M. Leatherwood, E. R. Lieber

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

A histamine-producing strain of Klebsiella pneumoniae was isolated from a sample of tuna sashimi implicated in an outbreak of scombroid fish poisoning. None of the other nine gram-negative bacterial strains isolated from the tuna sashimi was capable of equivalent histamine production. Bacterial histamine production was monitored in a tuna fish infusion broth (TFIB), and the implicated K. pneumoniae was capable of producing 442 mg of histamine per 100 g of tuna in TFIB in 7 h under controlled incubation conditions. Only 12 of 50 other K. pneumoniae strains, representing 5 distinct biochemical types, which had been originally isolated from foods, were able to produce such levels of histamine in TFIB. No correlation was found between histamine production and other biochemical characteristics or antibiotic resistance. Of the 12 histamine-producing strains, 11 belonged to type 2, which is characterized as indole negative with positive reactions in the urea and Voges-Proskauer tests. However, only 50% of the type 2 strains examined produced high levels of histamine in TFIB. Additionally, the implicated K. pneumoniae strain and one other strain belonged to type 1, which is characterized by positive reactions in the indole, urea, and Voges-Proskauer tests.

Original languageEnglish (US)
Pages (from-to)274-278
Number of pages5
JournalApplied and environmental microbiology
Volume37
Issue number2
StatePublished - Dec 1 1979

Fingerprint

Tuna
pneumonia
Klebsiella pneumoniae
histamine
poisoning
tuna
Poisoning
Histamine
Fishes
fish
urea
indoles
antibiotic resistance
Urea
incubation
Microbial Drug Resistance
food
Disease Outbreaks
testing
Food

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Histamine production by Klebsiella pneumoniae and an incident of scombroid fish poisoning. / Taylor, S. L.; Guthertz, L. S.; Leatherwood, M.; Lieber, E. R.

In: Applied and environmental microbiology, Vol. 37, No. 2, 01.12.1979, p. 274-278.

Research output: Contribution to journalArticle

Taylor, S. L. ; Guthertz, L. S. ; Leatherwood, M. ; Lieber, E. R. / Histamine production by Klebsiella pneumoniae and an incident of scombroid fish poisoning. In: Applied and environmental microbiology. 1979 ; Vol. 37, No. 2. pp. 274-278.
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