This work describes a high-resolution two-dimensional electrophoretic method for identification of bovine milk caseins. The isoelectric focusing separation was carried out in 100-jtL capillary tubes using high field strengths to achieve enhanced resolution. Subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed in a minigel format, and the result of the combined techniques gave enhanced protein resolution while requiring substantially less time than conventional two-dimensional methods. The procedure readily distinguished various isoforms of caseins, as well as phosphorylated forms of the proteins. The method was also used to demonstrate the effect of a proteinase derived from a Pseudomonas species on milk proteins.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)