High-Resolution Two-Dimensional Electrophoresis of Bovine Caseins

Michael G Zeece, Douglas L. Holt, Randy L. Wehling, Michael B. Liewen, Lynne R. Bush

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This work describes a high-resolution two-dimensional electrophoretic method for identification of bovine milk caseins. The isoelectric focusing separation was carried out in 100-jtL capillary tubes using high field strengths to achieve enhanced resolution. Subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed in a minigel format, and the result of the combined techniques gave enhanced protein resolution while requiring substantially less time than conventional two-dimensional methods. The procedure readily distinguished various isoforms of caseins, as well as phosphorylated forms of the proteins. The method was also used to demonstrate the effect of a proteinase derived from a Pseudomonas species on milk proteins.

Original languageEnglish (US)
Pages (from-to)378-383
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume37
Issue number2
DOIs
StatePublished - Mar 1 1989

Fingerprint

Caseins
Electrophoresis
electrophoresis
casein
Capillary tubes
Milk Proteins
cattle
Sodium Dodecyl Sulfate
Protein Isoforms
Proteins
Peptide Hydrolases
milk proteins
isoelectric focusing
Isoelectric Focusing
Pseudomonas
methodology
polyacrylamide gel electrophoresis
Polyacrylamide Gel Electrophoresis
Milk
proteinases

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Zeece, M. G., Holt, D. L., Wehling, R. L., Liewen, M. B., & Bush, L. R. (1989). High-Resolution Two-Dimensional Electrophoresis of Bovine Caseins. Journal of Agricultural and Food Chemistry, 37(2), 378-383. https://doi.org/10.1021/jf00086a023

High-Resolution Two-Dimensional Electrophoresis of Bovine Caseins. / Zeece, Michael G; Holt, Douglas L.; Wehling, Randy L.; Liewen, Michael B.; Bush, Lynne R.

In: Journal of Agricultural and Food Chemistry, Vol. 37, No. 2, 01.03.1989, p. 378-383.

Research output: Contribution to journalArticle

Zeece, MG, Holt, DL, Wehling, RL, Liewen, MB & Bush, LR 1989, 'High-Resolution Two-Dimensional Electrophoresis of Bovine Caseins', Journal of Agricultural and Food Chemistry, vol. 37, no. 2, pp. 378-383. https://doi.org/10.1021/jf00086a023
Zeece, Michael G ; Holt, Douglas L. ; Wehling, Randy L. ; Liewen, Michael B. ; Bush, Lynne R. / High-Resolution Two-Dimensional Electrophoresis of Bovine Caseins. In: Journal of Agricultural and Food Chemistry. 1989 ; Vol. 37, No. 2. pp. 378-383.
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