High-resolution mapping of tlt, a mouse mutant lacking otoconia

Huacheng C. Ying, Belén Hurlé, Yunxia W Lundberg, Barbara A. Bohne, Mary K. Wuerffel, David M. Ornitz

Research output: Contribution to journalArticle

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Abstract

The ability to sense gravity is enhanced by an extracellular structure that overlies the macular sensory epithelium. This complex consists of high density particles, otoconia, embedded within a gelatinous membrane. The tilted mouse specifically lacks otoconia, yet has no other detectable anatomic lesions. Furthermore, the penetrance of the tilted phenotype is nearly 100%. This mouse provides a model to identify genes that are involved in the development and function of vestibular otoconia. Using SSLP markers, we have mapped the tilted (tlt) gene on mouse Chromosome (Chr) 5 between D5Mit421 and D5Mit353/D5Mit128/D5Mit266/D5Mit267 by analysis of the progeny of an intersubspecific F2 intercross. We also mapped the fibroblast growth factor receptor 3 (Fgfr3) gene, a potential candidate for tlt, and the Huntington's disease homolog (Hdh) gene to D5Mit268, approximately 4.3 centiMorgans (cM) from the tilted locus. This study excludes both Fgfr3 and Hdh as candidate genes for tlt and identifies closely linked microsatellite markers that will be useful for the positional cloning of tlt.

Original languageEnglish (US)
Pages (from-to)544-548
Number of pages5
JournalMammalian Genome
Volume10
Issue number6
DOIs
StatePublished - Aug 26 1999

Fingerprint

Otolithic Membrane
Receptor, Fibroblast Growth Factor, Type 3
Genes
Huntington Disease
Chromosomes, Human, Pair 5
Aptitude
Penetrance
Gravitation
Microsatellite Repeats
Organism Cloning
Epithelium
Phenotype
Membranes

ASJC Scopus subject areas

  • Genetics

Cite this

Ying, H. C., Hurlé, B., Lundberg, Y. W., Bohne, B. A., Wuerffel, M. K., & Ornitz, D. M. (1999). High-resolution mapping of tlt, a mouse mutant lacking otoconia. Mammalian Genome, 10(6), 544-548. https://doi.org/10.1007/s003359901041

High-resolution mapping of tlt, a mouse mutant lacking otoconia. / Ying, Huacheng C.; Hurlé, Belén; Lundberg, Yunxia W; Bohne, Barbara A.; Wuerffel, Mary K.; Ornitz, David M.

In: Mammalian Genome, Vol. 10, No. 6, 26.08.1999, p. 544-548.

Research output: Contribution to journalArticle

Ying, HC, Hurlé, B, Lundberg, YW, Bohne, BA, Wuerffel, MK & Ornitz, DM 1999, 'High-resolution mapping of tlt, a mouse mutant lacking otoconia', Mammalian Genome, vol. 10, no. 6, pp. 544-548. https://doi.org/10.1007/s003359901041
Ying, Huacheng C. ; Hurlé, Belén ; Lundberg, Yunxia W ; Bohne, Barbara A. ; Wuerffel, Mary K. ; Ornitz, David M. / High-resolution mapping of tlt, a mouse mutant lacking otoconia. In: Mammalian Genome. 1999 ; Vol. 10, No. 6. pp. 544-548.
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