High phosphate (up to 600 mM) induces pseudohyphal development in five wild type Candida albicans

Jacob M. Hornby, Raluca Dumitru, Kenneth W. Nickerson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A method is described for the formation of nearly 100% pseudohyphae populations of wild-type Candida albicans A72. The method employs fungal growth at 37°C (ca. 5×106 cells/ml) in a glucose-proline-N-acetyl- glucosamine medium supplemented with up to 600 mM phosphate (KH 2PO4/K2HPO4 1:1) at pH 6.5. Four other strains of C. albicans (MEN, 10261, SG5314 and CAI-4) also formed pseudohyphae under these conditions, although the phosphate response profiles differed in the concentration required for each strain to form pseudohyphae.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalJournal of Microbiological Methods
Volume56
Issue number1
DOIs
StatePublished - Jan 2004

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carboxyamido-triazole
Candida albicans
Phosphates
Glucosamine
Proline
Glucose
Growth
Population
potassium phosphate

Keywords

  • Candida albicans
  • High phosphate
  • Pseudohyphae
  • Pseudomycelia

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

High phosphate (up to 600 mM) induces pseudohyphal development in five wild type Candida albicans. / Hornby, Jacob M.; Dumitru, Raluca; Nickerson, Kenneth W.

In: Journal of Microbiological Methods, Vol. 56, No. 1, 01.2004, p. 119-124.

Research output: Contribution to journalArticle

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