High-performance liquid chromatographic column-switching method for two cyclosporine metabolites in blood

Dennis J. Gmur, Patrick Meier, Gary C. Yee

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Abstract

Cyclosporine (CSA) is biotransformed to many metabolites which may contribute to its immunosuppressive and nephrotoxic activity. We report a rapid and sensitive, automated column-switching high-performance liquid chromatographic (HPLC) method for measuring CSA-M17 in whole blood; the method also separates CSA-M1. CSA metabolite standards were isolated by a preparative-scale HPLC method. Samples were prepared by protein precipitation with acetonitrile followed by dilution with water. CSA-M17 was initially separated on a C8 column; final separation was on a C18 column. The inter-day relative standard deviation at 50 ng/ml was 8% (n=3). Limit of detection was 20 ng/ml.

Original languageEnglish (US)
Pages (from-to)343-352
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume425
Issue numberC
DOIs
StatePublished - 1988

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ASJC Scopus subject areas

  • Chemistry(all)

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