High Frequency of Detection by PCR of Viral Nucleic Acid in The Blood of Infants Presenting with Clinical Myocarditis

Kathleen E. Simpson, Gregory A. Storch, Caroline K. Lee, Kent E. Ward, Saar Danon, Catherine M. Simon, Jeffrey W Delaney, Alan Tong, Charles E. Canter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Specific viruses are associated with pediatric myocarditis, but the prevalence of viral DNAemia detected by blood polymerase chain reaction (PCR) is unknown. We evaluated the prevalence of known cardiotropic viruses (enterovirus, adenovirus, human herpesvirus 6, and parvovirus B19) in children with clinical myocarditis (n = 21). Results were compared to pediatric controls with similar viral PCR testing. The majority of positive PCR (89 %) was noted in children ≤12 months of age at diagnosis compared to older children. Infant myocarditis patients (8/10) had increased the prevalence of PCR positivity compared to infant pediatric controls (4/114) (p < 0.0001). Other than age, patient characteristics at diagnosis were similar between PCR-positive and PCR-negative patients. Both PCR-negative myocarditis infants had clinical recovery at follow-up. Of the PCR-positive myocarditis infants, 4 had clinical recovery, 2 developed chronic cardiomyopathy, 1 underwent heart transplant, and 1 died. Infants with clinical myocarditis have a high rate of blood viral positivity, which is higher compared to older children with myocarditis and healthy infant controls. Age-related differences in PCR positivity may be due to differences in host and/or virus characteristics. Our findings suggest that viral blood PCR may be a useful diagnostic tool and identify patients who would potentially benefit from virus-specific therapy.

Original languageEnglish (US)
Pages (from-to)399-404
Number of pages6
JournalPediatric Cardiology
Volume37
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Myocarditis
Nucleic Acids
Polymerase Chain Reaction
Viruses
Pediatrics
Human Herpesvirus 6
Parvovirus
Enterovirus
Cardiomyopathies
Adenoviridae
Transplants

Keywords

  • DNAemia
  • Myocarditis
  • Pediatric cardiology

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Cardiology and Cardiovascular Medicine

Cite this

Simpson, K. E., Storch, G. A., Lee, C. K., Ward, K. E., Danon, S., Simon, C. M., ... Canter, C. E. (2016). High Frequency of Detection by PCR of Viral Nucleic Acid in The Blood of Infants Presenting with Clinical Myocarditis. Pediatric Cardiology, 37(2), 399-404. https://doi.org/10.1007/s00246-015-1290-6

High Frequency of Detection by PCR of Viral Nucleic Acid in The Blood of Infants Presenting with Clinical Myocarditis. / Simpson, Kathleen E.; Storch, Gregory A.; Lee, Caroline K.; Ward, Kent E.; Danon, Saar; Simon, Catherine M.; Delaney, Jeffrey W; Tong, Alan; Canter, Charles E.

In: Pediatric Cardiology, Vol. 37, No. 2, 01.02.2016, p. 399-404.

Research output: Contribution to journalArticle

Simpson, Kathleen E. ; Storch, Gregory A. ; Lee, Caroline K. ; Ward, Kent E. ; Danon, Saar ; Simon, Catherine M. ; Delaney, Jeffrey W ; Tong, Alan ; Canter, Charles E. / High Frequency of Detection by PCR of Viral Nucleic Acid in The Blood of Infants Presenting with Clinical Myocarditis. In: Pediatric Cardiology. 2016 ; Vol. 37, No. 2. pp. 399-404.
@article{849467fef598449e8becc38b393174b0,
title = "High Frequency of Detection by PCR of Viral Nucleic Acid in The Blood of Infants Presenting with Clinical Myocarditis",
abstract = "Specific viruses are associated with pediatric myocarditis, but the prevalence of viral DNAemia detected by blood polymerase chain reaction (PCR) is unknown. We evaluated the prevalence of known cardiotropic viruses (enterovirus, adenovirus, human herpesvirus 6, and parvovirus B19) in children with clinical myocarditis (n = 21). Results were compared to pediatric controls with similar viral PCR testing. The majority of positive PCR (89 {\%}) was noted in children ≤12 months of age at diagnosis compared to older children. Infant myocarditis patients (8/10) had increased the prevalence of PCR positivity compared to infant pediatric controls (4/114) (p < 0.0001). Other than age, patient characteristics at diagnosis were similar between PCR-positive and PCR-negative patients. Both PCR-negative myocarditis infants had clinical recovery at follow-up. Of the PCR-positive myocarditis infants, 4 had clinical recovery, 2 developed chronic cardiomyopathy, 1 underwent heart transplant, and 1 died. Infants with clinical myocarditis have a high rate of blood viral positivity, which is higher compared to older children with myocarditis and healthy infant controls. Age-related differences in PCR positivity may be due to differences in host and/or virus characteristics. Our findings suggest that viral blood PCR may be a useful diagnostic tool and identify patients who would potentially benefit from virus-specific therapy.",
keywords = "DNAemia, Myocarditis, Pediatric cardiology",
author = "Simpson, {Kathleen E.} and Storch, {Gregory A.} and Lee, {Caroline K.} and Ward, {Kent E.} and Saar Danon and Simon, {Catherine M.} and Delaney, {Jeffrey W} and Alan Tong and Canter, {Charles E.}",
year = "2016",
month = "2",
day = "1",
doi = "10.1007/s00246-015-1290-6",
language = "English (US)",
volume = "37",
pages = "399--404",
journal = "Pediatric Cardiology",
issn = "0172-0643",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - High Frequency of Detection by PCR of Viral Nucleic Acid in The Blood of Infants Presenting with Clinical Myocarditis

AU - Simpson, Kathleen E.

AU - Storch, Gregory A.

AU - Lee, Caroline K.

AU - Ward, Kent E.

AU - Danon, Saar

AU - Simon, Catherine M.

AU - Delaney, Jeffrey W

AU - Tong, Alan

AU - Canter, Charles E.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Specific viruses are associated with pediatric myocarditis, but the prevalence of viral DNAemia detected by blood polymerase chain reaction (PCR) is unknown. We evaluated the prevalence of known cardiotropic viruses (enterovirus, adenovirus, human herpesvirus 6, and parvovirus B19) in children with clinical myocarditis (n = 21). Results were compared to pediatric controls with similar viral PCR testing. The majority of positive PCR (89 %) was noted in children ≤12 months of age at diagnosis compared to older children. Infant myocarditis patients (8/10) had increased the prevalence of PCR positivity compared to infant pediatric controls (4/114) (p < 0.0001). Other than age, patient characteristics at diagnosis were similar between PCR-positive and PCR-negative patients. Both PCR-negative myocarditis infants had clinical recovery at follow-up. Of the PCR-positive myocarditis infants, 4 had clinical recovery, 2 developed chronic cardiomyopathy, 1 underwent heart transplant, and 1 died. Infants with clinical myocarditis have a high rate of blood viral positivity, which is higher compared to older children with myocarditis and healthy infant controls. Age-related differences in PCR positivity may be due to differences in host and/or virus characteristics. Our findings suggest that viral blood PCR may be a useful diagnostic tool and identify patients who would potentially benefit from virus-specific therapy.

AB - Specific viruses are associated with pediatric myocarditis, but the prevalence of viral DNAemia detected by blood polymerase chain reaction (PCR) is unknown. We evaluated the prevalence of known cardiotropic viruses (enterovirus, adenovirus, human herpesvirus 6, and parvovirus B19) in children with clinical myocarditis (n = 21). Results were compared to pediatric controls with similar viral PCR testing. The majority of positive PCR (89 %) was noted in children ≤12 months of age at diagnosis compared to older children. Infant myocarditis patients (8/10) had increased the prevalence of PCR positivity compared to infant pediatric controls (4/114) (p < 0.0001). Other than age, patient characteristics at diagnosis were similar between PCR-positive and PCR-negative patients. Both PCR-negative myocarditis infants had clinical recovery at follow-up. Of the PCR-positive myocarditis infants, 4 had clinical recovery, 2 developed chronic cardiomyopathy, 1 underwent heart transplant, and 1 died. Infants with clinical myocarditis have a high rate of blood viral positivity, which is higher compared to older children with myocarditis and healthy infant controls. Age-related differences in PCR positivity may be due to differences in host and/or virus characteristics. Our findings suggest that viral blood PCR may be a useful diagnostic tool and identify patients who would potentially benefit from virus-specific therapy.

KW - DNAemia

KW - Myocarditis

KW - Pediatric cardiology

UR - http://www.scopus.com/inward/record.url?scp=84959524428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959524428&partnerID=8YFLogxK

U2 - 10.1007/s00246-015-1290-6

DO - 10.1007/s00246-015-1290-6

M3 - Article

C2 - 26499513

AN - SCOPUS:84959524428

VL - 37

SP - 399

EP - 404

JO - Pediatric Cardiology

JF - Pediatric Cardiology

SN - 0172-0643

IS - 2

ER -