High c-Kit expression identifies hematopoietic stem cells with impaired self-renewal and megakaryocytic bias

Joseph Y. Shin, Wenhuo Hu, Mayumi Naramura, Christopher Y. Park

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Hematopoietic stem cells (HSCs) are heterogeneous with respect to their self-renewal, lineage, and reconstitution potentials. Although c-Kit is required for HSC function, gain and loss-of-function c-Kit mutants suggest that even small changes in c-Kit signaling profoundly affect HSC function. Herein, we demonstrate that even the most rigorously defined HSCs can be separated into functionally distinct subsets based on c-Kit activity. Functional and transcriptome studies show HSCs with low levels of surface c-Kit expression (c-Kitlo) and signaling exhibit enhanced self-renewal and long-term reconstitution potential compared with c-Kithi HSCs. Furthermore, c-Kitlo and c-Kithi HSCs are hierarchically organized, with c-Kithi HSCs arising from c-Kitlo HSCs. In addition, whereas c-Kithi HSCs give rise to long-term lymphomyeloid grafts, they exhibit an intrinsic megakaryocytic lineage bias. These functional differences between c-Kitlo and c-Kithi HSCs persist even under conditions of stress hematopoiesis induced by 5-fluorouracil. Finally, our studies show that the transition from c-Kitlo to c-Kithi HSC is negatively regulated by c-Cbl. Overall, these studies demonstrate that HSCs exhibiting enhanced self-renewal potential can be isolated based on c-Kit expression during both steady state and stress hematopoiesis. Moreover, they provide further evidence that the intrinsic functional heterogeneity previously described for HSCs extends to the megakaryocytic lineage.

Original languageEnglish (US)
Pages (from-to)217-231
Number of pages15
JournalJournal of Experimental Medicine
Volume211
Issue number2
DOIs
StatePublished - Feb 10 2014

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Hematopoietic Stem Cells
Hematopoiesis
Transcriptome
Fluorouracil

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

High c-Kit expression identifies hematopoietic stem cells with impaired self-renewal and megakaryocytic bias. / Shin, Joseph Y.; Hu, Wenhuo; Naramura, Mayumi; Park, Christopher Y.

In: Journal of Experimental Medicine, Vol. 211, No. 2, 10.02.2014, p. 217-231.

Research output: Contribution to journalArticle

Shin, Joseph Y. ; Hu, Wenhuo ; Naramura, Mayumi ; Park, Christopher Y. / High c-Kit expression identifies hematopoietic stem cells with impaired self-renewal and megakaryocytic bias. In: Journal of Experimental Medicine. 2014 ; Vol. 211, No. 2. pp. 217-231.
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