High-altitude ancestry and hypoxia acclimation have distinct effects on exercise capacity and muscle phenotype in deer mice

Mikaela A. Lui, Sajeni Mahalingam, Paras Patel, Alex D. Connaty, Catherine M. Ivy, Zachary A. Cheviron, Jay F Storz, Grant B. McClelland, Graham R. Scott

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Lui MA, Mahalingam S, Patel P, Connaty AD, Ivy CM, Cheviron ZA, Storz JF, McClelland GB, Scott GR. High-altitude ancestry and hypoxia acclimation have distinct effects on exercise capacity and muscle phenotype in deer mice. Am J Physiol Regul Integr Comp Physiol 308: R779–R791, 2015. First published February 18, 2015; doi:10.1152/ajpregu.00362.2014.—The hypoxic and cold environment at high altitudes requires that small mammals sustain high rates of O2 transport for exercise and thermogenesis while facing a diminished O2 availability. We used laboratory-born and -raised deer mice (Peromyscus maniculatus) from highland and lowland populations to determine the interactive effects of ancestry and hypoxia acclimation on exercise performance. Maximal O2 consumption (V O2 max) during exercise in hypoxia increased after hypoxia acclimation (equivalent to the hypoxia at ~4,300 m elevation for 6–8 wk) and was consistently greater in highlanders than in lowlanders. V O2max during exercise in normoxia was not affected by ancestry or acclimation. Highlanders also had consistently greater capillarity, oxidative fiber density, and maximal activities of oxidative enzymes (cytochrome c oxidase and citrate synthase) in the gastrocnemius muscle, lower lactate dehydrogenase activity in the gastrocnemius, and greater cytochrome c oxidase activity in the diaphragm. Hypoxia acclimation did not affect any of these muscle traits. The unique gastrocnemius phenotype of highlanders was associated with higher mRNA and protein abundances of peroxisome proliferator-activated receptor γ (PPARγ). Vascular endothelial growth factor (VEGFA) transcript abundance was lower in highlanders, and hypoxia acclimation reduced the expression of numerous genes that regulate angiogenesis and energy metabolism, in contrast to the observed population differences in muscle phenotype. Lowlanders exhibited greater increases in blood hemoglobin content, hematocrit, and wet lung mass (but not dry lung mass) than highlanders after hypoxia acclimation. Genotypic adaptation to high altitude, therefore, improves exercise performance in hypoxia by mechanisms that are at least partially distinct from those underlying hypoxia acclimation.

Original languageEnglish (US)
Pages (from-to)R779-R791
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume308
Issue number9
DOIs
StatePublished - Jan 1 2015

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Altitude Sickness
Peromyscus
Acclimatization
Phenotype
Muscles
Electron Transport Complex IV
Capillary Action
Hypoxia
Citrate (si)-Synthase
Peroxisome Proliferator-Activated Receptors
Thermogenesis
Pulmonary Edema
Diaphragm
Hematocrit
L-Lactate Dehydrogenase
Energy Metabolism
Vascular Endothelial Growth Factor A
Population
Mammals
Hemoglobins

Keywords

  • Capillarity
  • High-altitude adaptation
  • Oxidative capacity
  • Oxygen transport
  • PPAR<inf>Ɣ</inf>

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

High-altitude ancestry and hypoxia acclimation have distinct effects on exercise capacity and muscle phenotype in deer mice. / Lui, Mikaela A.; Mahalingam, Sajeni; Patel, Paras; Connaty, Alex D.; Ivy, Catherine M.; Cheviron, Zachary A.; Storz, Jay F; McClelland, Grant B.; Scott, Graham R.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 308, No. 9, 01.01.2015, p. R779-R791.

Research output: Contribution to journalArticle

Lui, Mikaela A. ; Mahalingam, Sajeni ; Patel, Paras ; Connaty, Alex D. ; Ivy, Catherine M. ; Cheviron, Zachary A. ; Storz, Jay F ; McClelland, Grant B. ; Scott, Graham R. / High-altitude ancestry and hypoxia acclimation have distinct effects on exercise capacity and muscle phenotype in deer mice. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2015 ; Vol. 308, No. 9. pp. R779-R791.
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