Hfq regulates antibacterial antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11

Gaoge Xu, Yuxin Zhao, Liangcheng Du, Guoliang Qian, Fengquan Liu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Lysobacter enzymogenes is an important biocontrol agent with the ability to produce a variety of lytic enzymes and novel antibiotics. Little is known about their regulatory mechanisms. Understanding these will be helpful for improving biocontrol of crop diseases and potential medical application. In the present study, we generated an hfq (encoding a putative ribonucleic acid chaperone) deletion mutant, and then utilized a new genomic marker-free method to construct an hfq-complemented strain. We showed for the first time that Hfq played a pleiotropic role in regulating the antibacterial antibiotic biosynthesis and extracellular lytic enzyme activity in L.enzymogenes. Mutation of hfq significantly increased the yield of WAP-8294A2 (an antibacterial antibiotic) as well as the transcription of its key biosynthetic gene, waps1. However, inactivation of hfq almost abolished the extracellular chitinase activity and remarkably decreased the activity of both extracellular protease and cellulase in L.enzymogenes. We further showed that the regulation of hfq in extracellular chitinase production was in part through the impairment of the secretion of chitinase A. Collectively, our results reveal the regulatory roles of hfq in antibiotic metabolite and extracellular lytic enzymes in the underexplored genus of Lysobacter.

Original languageEnglish (US)
Pages (from-to)499-509
Number of pages11
JournalMicrobial Biotechnology
Volume8
Issue number3
DOIs
StatePublished - May 1 2015

Fingerprint

Lysobacter
Biosynthesis
Antibiotics
Chitinases
Enzymes
Biocontrol
Anti-Bacterial Agents
Cellulase
Enzyme activity
Medical applications
Transcription
Metabolites
RNA
Crops
Peptide Hydrolases
Genes
Mutation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Hfq regulates antibacterial antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11. / Xu, Gaoge; Zhao, Yuxin; Du, Liangcheng; Qian, Guoliang; Liu, Fengquan.

In: Microbial Biotechnology, Vol. 8, No. 3, 01.05.2015, p. 499-509.

Research output: Contribution to journalArticle

@article{d571560094f64e54a5c96acea42be4fa,
title = "Hfq regulates antibacterial antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11",
abstract = "Lysobacter enzymogenes is an important biocontrol agent with the ability to produce a variety of lytic enzymes and novel antibiotics. Little is known about their regulatory mechanisms. Understanding these will be helpful for improving biocontrol of crop diseases and potential medical application. In the present study, we generated an hfq (encoding a putative ribonucleic acid chaperone) deletion mutant, and then utilized a new genomic marker-free method to construct an hfq-complemented strain. We showed for the first time that Hfq played a pleiotropic role in regulating the antibacterial antibiotic biosynthesis and extracellular lytic enzyme activity in L.enzymogenes. Mutation of hfq significantly increased the yield of WAP-8294A2 (an antibacterial antibiotic) as well as the transcription of its key biosynthetic gene, waps1. However, inactivation of hfq almost abolished the extracellular chitinase activity and remarkably decreased the activity of both extracellular protease and cellulase in L.enzymogenes. We further showed that the regulation of hfq in extracellular chitinase production was in part through the impairment of the secretion of chitinase A. Collectively, our results reveal the regulatory roles of hfq in antibiotic metabolite and extracellular lytic enzymes in the underexplored genus of Lysobacter.",
author = "Gaoge Xu and Yuxin Zhao and Liangcheng Du and Guoliang Qian and Fengquan Liu",
year = "2015",
month = "5",
day = "1",
doi = "10.1111/1751-7915.12246",
language = "English (US)",
volume = "8",
pages = "499--509",
journal = "Microbial Biotechnology",
issn = "1751-7907",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - Hfq regulates antibacterial antibiotic biosynthesis and extracellular lytic-enzyme production in Lysobacter enzymogenes OH11

AU - Xu, Gaoge

AU - Zhao, Yuxin

AU - Du, Liangcheng

AU - Qian, Guoliang

AU - Liu, Fengquan

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Lysobacter enzymogenes is an important biocontrol agent with the ability to produce a variety of lytic enzymes and novel antibiotics. Little is known about their regulatory mechanisms. Understanding these will be helpful for improving biocontrol of crop diseases and potential medical application. In the present study, we generated an hfq (encoding a putative ribonucleic acid chaperone) deletion mutant, and then utilized a new genomic marker-free method to construct an hfq-complemented strain. We showed for the first time that Hfq played a pleiotropic role in regulating the antibacterial antibiotic biosynthesis and extracellular lytic enzyme activity in L.enzymogenes. Mutation of hfq significantly increased the yield of WAP-8294A2 (an antibacterial antibiotic) as well as the transcription of its key biosynthetic gene, waps1. However, inactivation of hfq almost abolished the extracellular chitinase activity and remarkably decreased the activity of both extracellular protease and cellulase in L.enzymogenes. We further showed that the regulation of hfq in extracellular chitinase production was in part through the impairment of the secretion of chitinase A. Collectively, our results reveal the regulatory roles of hfq in antibiotic metabolite and extracellular lytic enzymes in the underexplored genus of Lysobacter.

AB - Lysobacter enzymogenes is an important biocontrol agent with the ability to produce a variety of lytic enzymes and novel antibiotics. Little is known about their regulatory mechanisms. Understanding these will be helpful for improving biocontrol of crop diseases and potential medical application. In the present study, we generated an hfq (encoding a putative ribonucleic acid chaperone) deletion mutant, and then utilized a new genomic marker-free method to construct an hfq-complemented strain. We showed for the first time that Hfq played a pleiotropic role in regulating the antibacterial antibiotic biosynthesis and extracellular lytic enzyme activity in L.enzymogenes. Mutation of hfq significantly increased the yield of WAP-8294A2 (an antibacterial antibiotic) as well as the transcription of its key biosynthetic gene, waps1. However, inactivation of hfq almost abolished the extracellular chitinase activity and remarkably decreased the activity of both extracellular protease and cellulase in L.enzymogenes. We further showed that the regulation of hfq in extracellular chitinase production was in part through the impairment of the secretion of chitinase A. Collectively, our results reveal the regulatory roles of hfq in antibiotic metabolite and extracellular lytic enzymes in the underexplored genus of Lysobacter.

UR - http://www.scopus.com/inward/record.url?scp=84928061647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928061647&partnerID=8YFLogxK

U2 - 10.1111/1751-7915.12246

DO - 10.1111/1751-7915.12246

M3 - Article

C2 - 25683974

AN - SCOPUS:84928061647

VL - 8

SP - 499

EP - 509

JO - Microbial Biotechnology

JF - Microbial Biotechnology

SN - 1751-7907

IS - 3

ER -