Heterogeneity of intron presence or absence in rDNA genes of the lichen species Physcia aipolia and P. stellaris

Dawn M. Simon, Cora L. Hummel, Sara L. Sheeley, Debashish Bhattacharya

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Intron origin and evolution are of high interest, yet the rates of insertion and loss are unclear. To investigate their spread, we studied ribosomal (r)DNA introns from the closely related lichens Physcia aipolia and P. stellaris. Both taxa are replete with rDNA spliceosomal introns and autocatalytic group I introns, many of which show presence/ absence polymorphism when screened with the PCR approach. This initially suggested that Physcia could be a model for studying intron retention and loss. However, during the course of a population-level analysis, we discovered widespread intron presence/absence heterogeneity within lichen thalli. To address this result, we sequenced multiple clones encoding nuclear rDNA and the single-copy elongation factor-1α (EF-1α) from individual thalli. These data showed extensive rDNA heterogeneity within individuals, rather than the presence of multiple fungi within a thallus. Our results suggest that considerable care must be taken when interpreting intron presence/absence in lichen rDNA, an observation that has general implications for the study of rDNA intron evolution.

Original languageEnglish (US)
Pages (from-to)389-399
Number of pages11
JournalCurrent Genetics
Volume47
Issue number6
DOIs
StatePublished - Jun 1 2005

Fingerprint

Lichens
Ribosomal DNA
Introns
Genes
Peptide Elongation Factor 1
Fungi
Clone Cells
Observation
Polymerase Chain Reaction

Keywords

  • Group I intron
  • Intron loss
  • Lichen
  • Physcia
  • Spliceosomal intron
  • rDNA

ASJC Scopus subject areas

  • Genetics

Cite this

Heterogeneity of intron presence or absence in rDNA genes of the lichen species Physcia aipolia and P. stellaris. / Simon, Dawn M.; Hummel, Cora L.; Sheeley, Sara L.; Bhattacharya, Debashish.

In: Current Genetics, Vol. 47, No. 6, 01.06.2005, p. 389-399.

Research output: Contribution to journalArticle

Simon, Dawn M. ; Hummel, Cora L. ; Sheeley, Sara L. ; Bhattacharya, Debashish. / Heterogeneity of intron presence or absence in rDNA genes of the lichen species Physcia aipolia and P. stellaris. In: Current Genetics. 2005 ; Vol. 47, No. 6. pp. 389-399.
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