Heterodimeric capping protein is required for stereocilia length and width regulation

Matthew R. Avenarius, Jocelyn F. Krey, Rachel A. Dumont, Clive P. Morgan, Connor B. Benson, Sarath Vijayakumar, Christopher L. Cunningham, Deborah I. Scheffer, David P. Corey, Ulrich Müller, Sherri M Jones, Peter G. Barr-Gillespie

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Control of the dimensions of actin-rich processes like filopodia, lamellipodia, microvilli, and stereocilia requires the coordinated activity of many proteins. Each of these actin structures relies on heterodimeric capping protein (CAPZ), which blocks actin polymerization at barbed ends. Because dimension control of the inner ear's stereocilia is particularly precise, we studied the CAP ZB subunit in hair cells. CAP ZB, present at ~100 copies per stereocilium, concentrated at stereocilia tips as hair cell development progressed, similar to the CAP ZB-interacting protein TWF2. We deleted Capzb specifically in hair cells using Atoh1-Cre, which eliminated auditory and vestibular function. Capzb-null stereocilia initially developed normally but later shortened and disappeared; surprisingly, stereocilia width decreased concomitantly with length. CAP ZB2 expressed by in utero electroporation prevented normal elongation of vestibular stereocilia and irregularly widened them. Together, these results suggest that capping protein participates in stereocilia widening by preventing newly elongating actin filaments from depolymerizing.

Original languageEnglish (US)
Pages (from-to)3861-3881
Number of pages21
JournalJournal of Cell Biology
Volume216
Issue number11
DOIs
StatePublished - Nov 1 2017

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Stereocilia
Proteins
Actins
Pseudopodia
Electroporation
Inner Ear
Microvilli
Actin Cytoskeleton
Polymerization

ASJC Scopus subject areas

  • Cell Biology

Cite this

Avenarius, M. R., Krey, J. F., Dumont, R. A., Morgan, C. P., Benson, C. B., Vijayakumar, S., ... Barr-Gillespie, P. G. (2017). Heterodimeric capping protein is required for stereocilia length and width regulation. Journal of Cell Biology, 216(11), 3861-3881. https://doi.org/10.1083/jcb.201704171

Heterodimeric capping protein is required for stereocilia length and width regulation. / Avenarius, Matthew R.; Krey, Jocelyn F.; Dumont, Rachel A.; Morgan, Clive P.; Benson, Connor B.; Vijayakumar, Sarath; Cunningham, Christopher L.; Scheffer, Deborah I.; Corey, David P.; Müller, Ulrich; Jones, Sherri M; Barr-Gillespie, Peter G.

In: Journal of Cell Biology, Vol. 216, No. 11, 01.11.2017, p. 3861-3881.

Research output: Contribution to journalArticle

Avenarius, MR, Krey, JF, Dumont, RA, Morgan, CP, Benson, CB, Vijayakumar, S, Cunningham, CL, Scheffer, DI, Corey, DP, Müller, U, Jones, SM & Barr-Gillespie, PG 2017, 'Heterodimeric capping protein is required for stereocilia length and width regulation', Journal of Cell Biology, vol. 216, no. 11, pp. 3861-3881. https://doi.org/10.1083/jcb.201704171
Avenarius, Matthew R. ; Krey, Jocelyn F. ; Dumont, Rachel A. ; Morgan, Clive P. ; Benson, Connor B. ; Vijayakumar, Sarath ; Cunningham, Christopher L. ; Scheffer, Deborah I. ; Corey, David P. ; Müller, Ulrich ; Jones, Sherri M ; Barr-Gillespie, Peter G. / Heterodimeric capping protein is required for stereocilia length and width regulation. In: Journal of Cell Biology. 2017 ; Vol. 216, No. 11. pp. 3861-3881.
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